A fluorescent DNA probe
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Hoechst 33342 (hydrochloride)

Item No. 15547

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
2’-(4-ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5’-bi-1H-benzimidazole, trihydrochloride
CAS Number
875756-97-1
Synonyms
  • Bisbenzimide
  • HOE 33342
  • NSC 334072
Molecular Formula
C27H28N6O • 3HCl
Formula Weight
561.9
Purity
≥98%
Emission
461 nm
Excitation
350 nm
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMSO: 20 mg/mlPBS (pH 7.2): 5 mg/ml
λmax
239, 272, 355 nm
SMILES
CN1CCN(C2=CC(NC(C3=CC=C(N=C(C4=CC=C(OCC)C=C4)N5)C5=C3)=N6)=C6C=C2)CC1.Cl.Cl.Cl
InChi Code
InChI=1S/C27H28N6O.3ClH/c1-3-34-21-8-4-18(5-9-21)26-28-22-10-6-19(16-24(22)30-26)27-29-23-11-7-20(17-25(23)31-27)33-14-12-32(2)13-15-33;;;/h4-11,16-17H,3,12-15H2,1-2H3,(H,28,30)(H,29,31);3*1H
InChi Key
JABNPSKWVNCGMX-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    25 mg

    $40

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    50 mg

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    100 mg

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    500 mg

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    Product Description

    Hoechst 33342 is a cell-permeable, benzimidazole dye that stains DNA by binding to the minor groove of adenine and thymine-rich sequences.1 It emits blue fluorescence (excitation 350 nm/emission maximum 461 nm) when bound to double stranded DNA and is useful as a marker of nuclei for cell cycle studies and to distinguish nuclear morphology in apoptotic cells.2,3,4

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Sabnis, R.W. Handbook of biological dyes and stains: Synthesis and industrial applications. (2010).

    2. Burres, N.S., Frigo, A., Rasmussen, R.R., et al. A colorimetric microassay for the detection of agents that interact with DNA. J. Nat. Prod. 55(11), 1582-1587 (1992).

    3. Lalande, M.E., Ling, V., and Miller, R.G. Hoechst 33342 dye uptake as a probe of membrane permeability changes in mammalian cells. Proc. Natl. Acad. Sci. USA 78(1), 363-367 (1981).