A fluorescent DNA probe
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Alternative(s)
15547Hoechst 33342 (hydrochloride)
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Hoechst 33342 (hydrochloride) (solution)

Item No. 40797

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
2′-(4-ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5′-bi-1H-benzimidazole, trihydrochloride
CAS Number
875756-97-1
Synonyms
  • Bisbenzimide
Molecular Formula
C27H28N6O • 3HCl
Formula Weight
452.6
Purity
≥98%
Emission
465 nm
Excitation
350 nm
Formulation (Request formulation change)
A 20 mM solution in water
Solubility(Learn about Variance in Solubility)
Water: Sparingly soluble: 1-10mg/ml
SMILES
CN1CCN(C2=CC(NC(C3=CC=C(N=C(C4=CC=C(OCC)C=C4)N5)C5=C3)=N6)=C6C=C2)CC1.Cl.Cl.Cl
InChi Code
InChI=1S/C27H28N6O.3ClH/c1-3-34-21-8-4-18(5-9-21)26-28-22-10-6-19(16-24(22)30-26)27-29-23-11-7-20(17-25(23)31-27)33-14-12-32(2)13-15-33;;;/h4-11,16-17H,3,12-15H2,1-2H3,(H,28,30)(H,29,31);3*1H
InChi Key
JABNPSKWVNCGMX-UHFFFAOYSA-N
Shipping & Storage Information
Storage
4°C
Shipping
Wet ice in continental US; may vary elsewhere
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    2 ml

    $55

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    5 ml

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    Product Description

    Hoechst 33342 is a fluorescent DNA probe.1 It forms a fluorescent complex by binding to the minor groove of adenine and thymine-rich sequences.1 Hoechst 33342 displays an emission maximum of 465 when bound to dsDNA upon excitation at 350 nm.2 It has been used as a marker of nuclei in cell cycle studies and to distinguish nuclear morphology in apoptotic cells.3,4,5

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Sabnis, R.W. Handbook of biological dyes and stains: Synthesis and industrial applications. (2010).

    2. Latt, S.A., and Stetten, G. Spectral studies on 33258 Hoechst and related bisbenzimidazole dyes useful for fluorescent detection of deoxyribonucleic acid synthesis. J. Histochem. Cytochem. 24(1), 24-33 (1976).

    3. Burres, N.S., Frigo, A., Rasmussen, R.R., et al. A colorimetric microassay for the detection of agents that interact with DNA. J. Nat. Prod. 55(11), 1582-1587 (1992).

    4. Lalande, M.E., Ling, V., and Miller, R.G. Hoechst 33342 dye uptake as a probe of membrane permeability changes in mammalian cells. Proc. Natl. Acad. Sci. USA 78(1), 363-367 (1981).