For immunochemical detection of HHATL
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HHATL Polyclonal Antibody

Item No. 15648

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • C3orf3
  • Glycerol Uptake Transporter Homolog
  • GUP1
  • Hedgehog Acyltransferase-like Protein
  • KIAA1173
  • MBOAT3
  • MSTP002
  • OACT3
  • Protein-Cysteine N-Palmitoyltransferase HHAT-like Protein
Immunogen
Synthetic peptide from the C-terminal region of human HHAT
MW
~60 kDa
Formulation (Request formulation change)
500 µl of peptide affinity-purified polyclonal antibody
Storage Buffer
PBS, pH 7.2, with 50% glycerol and 0.02% sodium azide
Host
Rabbit
Applications
FC, IF, and WB
Species Reactivity
(+) Human HHATL
UniProt Accession №
Q9HCP6
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    1 ea (500 µl)

    $401

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    Cayman Chemical
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    Product Description

    Hedgehog acyltransferase-like (HHATL) is the mammalian homolog of yeast Gup1 that is encoded by the HHATL gene in humans.1 HHATL is a membrane-bound O-acyltransferase (MBOAT) superfamily member and contains multiple transmembrane domains common to this family but lacks acyltransferase function due to a single amino acid substitution of histidine for leucine at position 447.1,2 It is localized to the endoplasmic reticulum (ER) and is expressed in the heart, skeletal muscle, and brain.1,3,4 HHATL colocalizes with sonic hedgehog (Shh) and is a negative regulator of Shh N-terminal palmitoylation, a post-translational modification that is critical for Shh signaling in neural development and embryogenesis.3 Hhatl-/- neonatal mice fail to develop proper suckling ability, leading to malnutrition and death by postnatal day 14.5 HHATL expression is decreased in six nasopharyngeal carcinoma cell lines, as well as in tissue isolated from patients with nasopharyngeal carcinoma or skin squamous cell carcinoma.6,7 Cayman's HHATL Polyclonal Antibody can be used for flow cytometry (FC), immunofluorescence (IF), and Western blot applications. The antibody recognizes HHATL at ~60 kDa from human samples.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Lucas, C., Ferreira, C., Cazzanelli, G., et al. Yeast Gup1(2) proteins are homologues of the hedgehog morphogens acyltransferases HHAT(L): Facts and implications. J. Dev. Biol. 4(4), 33 (2016).

    2. Chang, C.C.Y., Sun, J., and Chang, T.Y. Membrane-bound O-acyltransferases (MBOATs). Front. Biol. 6(3), 177-182 (2011).

    3. Abe, Y., Kita, Y., and Niikura, T. Mammalian Gup1, a homolog of Saccharomyces cerevisiae glycerol uptake/transporter 1, acts as a negative regulator for N-terminal palmitoylation of Sonic hedgehog. FEBS J. 275(2), 318-331 (2008).

    4. Bleve, G., Zacheo, G., Cappello, M.S., et al. Subcellular localization and functional expression of the glycerol uptake protein 1 (GUP1) of Saccharomyces cerevisiae tagged with green fluorescent protein. Biochem. J. 390(Pt. 1), 145-155 (2005).

    5. Van, B., Nishi, M., Komazaki, S., et al. Mitsugumin 56 (hedgehog acyltransferase-like) is a sarcoplasmic reticulum-resident protein essential for postnatal muscle maturation. FEBS Lett. 589(10), 1095-1104 (2015).

    6. Zhang, S.-Q., Peng, H., Song, L.-Y., et al. Detection of KIAA1173 gene expression in nasopharyngeal carcinoma tissues and cell lines on tissue microarray. Ai Zheng 24(11), 1322-1326 (2005).

    7. Zhang, S.-Q., Tian, X., Luo, Y.-W., et al. Expression, clinical and pathological significance of KIAA1173 gene in skin squamous cell carcinoma. Zhonghua Yi Xue Za Zhi 90(18), 1243-1246 (2010).