For immunodetection of sPLA2 (human Type IIA)
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sPLA2 (human Type IIA) Polyclonal Antiserum

Item No. 160502

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • GIIC sPLA2
  • Group IIA Phospholipase A2
  • NPS-PLA2
  • Phosphatidylcholine 2-Acylhydrolase 2A
  • Phospholipase A2 Membrane Associated
Immunogen
Human recombinant synovial sPLA2 (Type IIA)
Formulation (Request formulation change)
100 µl of Lyophilized antiserum
Storage Buffer
Lyophilized from serum. Reconstitute in 100 µl of double distilled water
Host
Rabbit
Applications
WB
Cross Reactivity
(-) sPLA2 (Type V)
Species Reactivity
(+) Human(-) Bee venom
UniProt Accession №
P14555
Origin
Animal/Rabbit
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    Product Description

    sPLA2 catalyzes the hydrolysis of phospholipids at the sn-2 position yielding a free fatty acid and a lysophospholipid.1 The release of arachidonic acid from membrane phospholipids by PLA2 is believed to be a key step in the control of eicosanoid production within the cell.2 Human sPLA2 (Type IIA) is secreted, 14 kDa protein which is dependent on Ca2+ for optimal activity. Human sPLA2 (Type IIA) is 79% homologous to rat platelet and murine Type II sPLA2s. Cayman's sPLA2 antibody detects a band of approximately 15 kD by Western blot. For other applications, the working concentration of the antiserum must be determined empirically

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Dennis, E.A. Phospholipases. The Enzymes XVI, (1983).

    2. Dennis, E.A. Diversity of group types, regulation, and function of phospholipase A2. The Journal of Biological Chemisty 269(18), 13057-13060 (1994).