ARN1203

Arachidonoyl ethanolamide (AEA, Item No. 90050), also known as anandamide, is an endogenous ligand of the cannabinoid receptors.¹ AEA is metabolized by fatty acid amide hydrolase (FAAH) to give arachidonic acid (Item No. 90010) and ethanolamine.² ARN1203 is a fluorinated analog of AEA that serves as a substrate of FAAH (K_m = 29 µM).³ Fluorinated substrates, like ARN1023, are used in 'n-fluorine atoms for biochemical screening' (n-FABS) assays, NMR functional assays that directly measure the conversion of the substrate into product.⁴ ARN1203 is used to perform n-FABS against FAAH to identify novel inhibitors.^3,5,6

WARNING This product is not for human or veterinary use.

1. Felder, C.C., Briley, E.M., Axelrod, J., et al. Anandamide, an endogenous cannabimimetic eicosanoid, binds to the cloned human cannabinoid receptor and stimulates receptor-mediated signal transduction. Proc. Natl. Acad. Sci. USA 90(16), 7656-7660 (1993).

2. Felder, C.C., Dickason-Chesterfield, A.K., and Moore, S.A. Cannabinoid biology: The search for new therapeutic targets. Mol. Interv. 6(3), 149-161 (2006).

3. Lambruschini, C., Veronesi, M., Romeo, E., et al. Development of fragment-based n-FABS NMR screening applied to the membrane enzyme FAAH. Chembiochem 14(13), 1611-1619 (2013).

4. Veronesi, M., Romeo, E., Lambruschini, C., et al. Fluorine NMR-based screening on cell membrane extracts. ChemMedChem 9(2), 286-289 (2014).

5. Bertolacci, L., Romeo, E., Veronesi, M., et al. A binding site for nonsteroidal anti-inflammatory drugs in fatty acid amide hydrolase. J. Am. Chem. Soc. 135(1), 22-25 (2013).