A general inhibitor of DUBs
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PR-619

Item No. 16276

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
thiocyanic acid, C,C'-(2,6-diamino-3,5-pyridinediyl) ester
CAS Number
2645-32-1
Synonyms
  • 2,6-Diamino-3,5-dithiocyanopyridine
  • DUB Inhibitor V
Molecular Formula
C7H5N5S2
Formula Weight
223.3
Purity
≥98%
Formulation
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 10 mg/mlDMSO: 20 mg/mlDMSO:PBS(pH7.2) (1:1): 0.5 mg/ml
λmax
220, 266, 320 nm
SMILES
NC1=NC(N)=C(SC#N)C=C1SC#N
InChi Code
InChI=1S/C7H5N5S2/c8-2-13-4-1-5(14-3-9)7(11)12-6(4)10/h1H,(H4,10,11,12)
InChi Key
ZXOBLNBVNROVLC-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    5 mg

    $65

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    10 mg

    $115

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    25 mg

    $172

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    50 mg

    $308

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    Product Description

    PR-619 is a general inhibitor of deubiquitylating enzymes (DUBs), significantly reducing the activity of a panel of DUBs with EC50 values ranging from 5-20 µM.1 It is cell-permeable and reversible in its action.1 It is selective for DUBs over other proteases.1 PR-619 induces the accumulation of polyubiquitinated proteins in cells, including those not involved in the ubiquitin-proteasome system.2 It is used to study the role of DUBs in diverse pathways, including protein turnover and neurodegeneration.3,4,5

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Altun, M., Kramer, H.B., Willems, L.I., et al. Activity-based chemical proteomics accelerates inhibitor development for deubiquitylating enzymes. Chem. Biol. 18(11), 1401-1412 (2011).

    2. Udeshi, N.D., Mani, D.R., Eisenhaure, T., et al. Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition. Mol. Cell. Proteomics 11(15), 148-159 (2012).

    3. Balut, C.M., Loch, C.M., and Devor, D.C. Role of ubiquitylation and USP8-dependent deubiquitylation in the endocytosis and lysosomal targeting of plasma membrane KCa3.1. The FASEB Journal 25(11), 3938-3948 (2011).

    4. Seiberlich, V., Goldbaum, O., Zhukareva, V., et al. The small molecule inhibitor PR-619 of deubiquitinating enzymes affects the microtubule network and causes protein aggregate formation in neural cells: Implications for neurodegenerative diseases. Biochim. Biophys. Acta 1823(11), 2057-2068 (2012).

    5. Bertuccio, C.A., Lee, S.L., Wu, G., et al. Anterograde trafficking of KCa3.1 in polarized epithelia is Rab1- and Rab8-dependent and recycling endosome-independent. PLoS One 9(3), 1-16 (2014).