A chromogenic substrate for β-galactosidase
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Isomer(s)
36095X-α-Gal
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X-Gal

Item No. 16495

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
5-bromo-4-chloro-1H-indol-3-yl-β-D-galactopyranoside
CAS Number
7240-90-6
Synonyms
  • BCIG
  • X-β-Gal
  • 5-bromo-4-chloro-3-indolyl-β-D-Galactopyranoside
Molecular Formula
C14H15BrClNO6
Formula Weight
408.6
Purity
≥98%
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 30 mg/mlDMSO: 30 mg/mlDMSO:PBS(pH7.2) (1:1): 0.5 mg/ml
λmax
233, 292 nm
SMILES
O[C@H]1[C@H](OC2=CNC3=C2C(Cl)=C(Br)C=C3)O[C@H](CO)[C@H](O)[C@@H]1O
InChi Code
InChI=1S/C14H15BrClNO6/c15-5-1-2-6-9(10(5)16)7(3-17-6)22-14-13(21)12(20)11(19)8(4-18)23-14/h1-3,8,11-14,17-21H,4H2/t8-,11+,12+,13-,14-/m1/s1
InChi Key
OPIFSICVWOWJMJ-AEOCFKNESA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    1 g

    $54

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    5 g

    $149

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    10 g

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    Product Description

    X-Gal is a chromogenic substrate for β-galactosidase.1 Upon cleavage by β-galactosidase, a blue pigment is released that can be used as a marker of β-galactosidase activity. X-Gal has been used for the detection of lacZ activity in reporter assays and in the determination of protein-protein interactions.2,3

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Burn, S.F. Detection of β-galactosidase activity: X-gal staining. Methods Mol. Biol. 886, 241-250 (2012).

    2. Bout, A., Valerio, D., and Scholte, B.J. In vivo transfer and expression of the lacZ gene in the mouse lung. Exp. Lung Res. 19(2), 193-202 (1993).

    3. Möckli, N., and Auerbach, D. Quantitative β-galactosidase assay suitable for high-throughput applications in the yeast two-hybrid system. Biotechniques 36(5), 872-876 (2004).