A chromogenic substrate for α-galactosidase
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Isomer(s)
16495X-Gal
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X-α-Gal

Item No. 36095

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
5-bromo-4-chloro-1H-indol-3-yl α-D-galactopyranoside
CAS Number
107021-38-5
Synonyms
  • 5-bromo-4-chloro-3-indolyl-α-D-Galactopyranoside
Molecular Formula
C14H15BrClNO6
Formula Weight
408.6
Purity
≥98%
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMF: 1 mg/mlDMSO: 10 mg/mlEthanol: Slightly solublePBS (pH 7.2): 0.25 mg/ml
λmax
233 nm
SMILES
BrC1=CC=C2C(C(O[C@H]3O[C@@H]([C@@H]([C@@H]([C@H]3O)O)O)CO)=CN2)=C1Cl
InChi Code
InChI=1S/C14H15BrClNO6/c15-5-1-2-6-9(10(5)16)7(3-17-6)22-14-13(21)12(20)11(19)8(4-18)23-14/h1-3,8,11-14,17-21H,4H2/t8-,11+,12+,13-,14+/m1/s1
InChi Key
OPIFSICVWOWJMJ-YGEXGZRRSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    100 mg

    $62

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    250 mg

    $145

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    500 mg

    $274

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    1 g

    $395

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    Product Description

    X-α-Gal is a chromogenic substrate for α-galactosidase.1 Upon cleavage by α-galactosidase, a blue pigment is released that can be used as a marker of α-galactosidase activity. X-α-Gal has been used in the identification of S. cerevisiae and S. bayanus hybrid yeast.2

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Goddard, A., Ladds, G., and Davey, J. Development of a semi-quantitative plate-based α-galactosidase gene reporter for Schizosaccharomyces pombe and its use to isolate a constitutively active Mam2. Yeast 22(1), 31-41 (2005).

    2. Sato, M., Kishimoto, M., Watari, J., et al. Breeding of brewer’s yeast by hybridization between a top-fermenting yeast Saccharomyces cerevisiae and a cryophilic yeast Saccharomyces bayanus. J. Biosci. Bioeng. 93(5), 509-511 (2002).