A chemiluminescent probe
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Isomer(s)
45126Isoluminol
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Luminol

Item No. 16803

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
5-amino-2,3-dihydro-1,4-phthalazinedione
CAS Number
521-31-3
Synonyms
  • 3-Aminophthalhydrazide
  • NSC 5064
Molecular Formula
C8H7N3O2
Formula Weight
177.2
Purity
≥98%
Emission
411 nm
Excitation
355 nm
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMSO: 2 mg/mlDMSO:PBS (pH 7.2) (1:10): 0.1 mg/ml
λmax
220, 295, 355 nm
SMILES
O=C(NN1)C2=C(C=CC=C2N)C1=O
InChi Code
InChI=1S/C8H7N3O2/c9-5-3-1-2-4-6(5)8(13)11-10-7(4)12/h1-3H,9H2,(H,10,12)(H,11,13)
InChi Key
HWYHZTIRURJOHG-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    5 g

    $49

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    10 g

    $86

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    25 g

    $190

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    50 g

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    Product Description

    Luminol is a cyclic diacyl hydrazide that exhibits chemiluminescence upon oxidation. For example, HRP, combined with hydrogen peroxide as an activator, causes luminescent peroxidation of luminol.1 This reaction can be enhanced by certain phenol derivatives, such as p-substituted phenols.2 Luminol can also be oxidized, and chemiluminesce, by compounds containing iron, copper, gold, and cyanide.3,4 The excitation/emission maxima for luminol are 355/411 nm.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Cormier, M.J., and Prichard, P.M. An investigation of the mechanism of the luminescent peroxidation of luminol by stopped flow techniques. The Journal of Biological Chemisty 243(18), 4706-4714 (1968).

    2. Thorpe, G.H.G., Kricka, L.J., Moseley, S.B., et al. Phenols as enhancers of the chemiluminescent horseradish peroxidase-luminol-hydrogen peroxide reaction: Application in luminescence-monitored enzyme immunoassays. Clin. Chem. 31(8), 1335-1341 (1985).

    3. Fan, A., Cao, Z., Li, H., et al. Chemiluminescence platforms in immunoassay and DNA analyses. Anal. Sci. 25(5), 587-597 (2009).

    4. Vladimirov, Y.A., and Proskurnina, E.V. Free radicals and cell chemiluminescence. Biochemistry (Mosc.) 74(13), 1545-1566 (2009).