A chemiluminescent probe
Related Products
Isomer(s)
16803Luminol
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Isoluminol

Item No. 45126

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
7-amino-4-hydroxy-1(2H)-phthalazinone
CAS Number
3682-14-2
Synonyms
  • NSC 13567
Molecular Formula
C8H7N3O2
Formula Weight
177.2
Purity
≥98%
Excitation
416 nm
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
Acetonitrile:Water (1:1): Slightly soluble: 0.1-1 mg/ml
SMILES
NC1=CC=C2C(C(NNC2=O)=O)=C1
InChi Code
InChI=1S/C8H7N3O2/c9-4-1-2-5-6(3-4)8(13)11-10-7(5)12/h1-3H,9H2,(H,10,12)(H,11,13)
InChi Key
HUDPLKWXRLNSPC-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

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    1 g

    $29

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    5 g

    $65

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    10 g

    $116

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    25 g

    $254

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    Product Description

    Isoluminol is a chemiluminescent probe and an isomer of luminol (Item No. 16803).1,2 It displays a chemiluminescent emission maximum of 416 nm.1 Isoluminol exhibits enhanced chemiluminescence when conjugated to proteins and antibodies and has been used in competitive protein binding assays and immunoassays.1,3,4 It has also been used to measure the production of extracellular reactive oxygen species (ROS).5

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Brundrett, R.B., and White, E.H. Synthesis and chemiluminescence of derivatives of luminol and isoluminol. J. Am. Chem. Soc. 96(24), 7497-7502 (1976).

    2. Schroeder, H.R., and Yeager, F.M. Chemiluminescence yields and detection limits of some isoluminol derivatives in various oxidation systems. Anal. Chem. 50(8), 1114-1120 (1978).

    3. Schroeder, H.R., Vogelhut, P.O., Carrico, R.J., et al. Competitive protein binding assay for biotin monitored by chemiluminescence. Anal. Chem. 48(13), 1933-1937 (1976).

    4. Messeri, G., Orlandini, A., and Pazzagli, M. Luminescent immunoassay using isoluminol derivatives. J. Biolumin. Chemilumin. 4(1), 154-158 (1989).

    5. Lundqvist, H., and Dahlgren, C. Isoluminol-enhanced chemiluminescence: A sensitive method to study the release of superoxide anion from human neutrophils. Free Rad. Biol. Med. 20(6), 785-792 (1996).