Latanoprost (free acid)

Latanoprost (free acid) is a derivative of prostaglandin F_2α (PGF_2α; Item Nos. 16010 | 16020), an FP receptor agonist, and an active metabolite of the prodrug latanoprost (Item No. 16812).¹ It selectively binds to the FP receptor (K_i = 0.098 µM) over the EP₁, EP₂, EP₃, and EP₄ receptors (K_is = 2.06, 39.667, 7.519, and 75 µM, respectively), as well as the DP, IP, and TP receptors (K_is = ≥20, ≥90, and ≥60 µM, respectively). It induces phosphoinositide turnover in isolated human ciliary muscle and human trabecular meshwork cells, mouse NIH3T3 fibroblasts, and rat A7r5 vascular smooth muscle cells, which all endogenously express FP receptors (EC₅₀s = 124, 35, 32, and 35 nM, respectively), as well as HEK293 cells expressing human ocular FP receptors (EC₅₀ = 45.7 nM). It also increases intracellular calcium levels in hEP₁-5/293-AEQ17 cells expressing EP₁ receptors in an aequorin-based calcium assay (EC₅₀ = 119 nM).² Latanoprost (free acid) induces contraction of isolated cat iris sphincter smooth muscle (EC₅₀ = 29.9 nM).³

WARNING This product is not for human or veterinary use.

1. Sharif, N.A., Kelly, C.R., Crider, J.Y., et al. Ocular hypotensive FP prostaglandin (PG) analogs: PG receptor subtype binding affinities and selectivities, and agonist potencies at FP and other PG receptors in cultured cells. J. Ocul. Pharmacol. Ther. 19(6), 501-515 (2003).

2. Ungrin, M.D., Carrière, M.C., Denis, D., et al. Key structural features of prostaglandin E₂ and prostanoid analogs involved in binding and activation of the human EP₁ prostanoid receptor. Mol. Pharmacol. 59(6), 1446-1456 (2001).

3. Sharif, N.A., Kaddour-Djebbar, I., and Abdel-Latif, A.A. Cat iris sphincter smooth-muscle contraction: Comparison of FP-class prostaglandin analog agonist activities. J. Ocul. Pharmacol. Ther. 24(2), 152-163 (2008).