A probe to detect H2O2 in mitochondria
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Labeled Version(s)
17470MitoB-d15
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MitoB

Item No. 17116

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
[(3-boronophenyl)methyl]triphenyl-phosphonium, monobromide
CAS Number
1247025-84-8
Synonyms
  • MitoBoronic Acid
Molecular Formula
C25H23BO2P • Br
Formula Weight
477.1
Purity
≥95%
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 5 mg/mlDMSO: 10 mg/mlDMSO:PBS (pH 7.2) (1:1): 0.5 mg/mlEthanol: 0.5 mg/ml
SMILES
OB(O)C1=CC=CC(C[P+](C2=CC=CC=C2)(C3=CC=CC=C3)C4=CC=CC=C4)=C1.[Br-]
InChi Code
InChI=1S/C25H23BO2P.BrH/c27-26(28)22-12-10-11-21(19-22)20-29(23-13-4-1-5-14-23,24-15-6-2-7-16-24)25-17-8-3-9-18-25;/h1-19,27-28H,20H2;1H/q+1;/p-1
InChi Key
VDNRROALFUZCBO-UHFFFAOYSA-M
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    5 mg

    $75

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    10 mg

    $134

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    50 mg

    $589

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    100 mg

    $1,030

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    Product Description

    MitoB is a ratiometric mass spectrometry probe that can be used for assessing changes in H2O2 within mitochondria in vivo. MitoB contains a triphenylphosphonium cation component that drives its accumulation in mitochondria where its arylboronic moiety selectively reacts with H2O2 to produce a phenol product, MitoP.1,2 Quantifying the MitoP/MitoB ratio by LC-MS/MS reflects the mitochondrial matrix H2O2 concentration.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Cochemé, H.M., Logan, A., Prime, T.A., et al. Using the mitochondria-targeted ratiometric mass spectrometry probe MitoB to measure H2O2 in living Drosophila. Nat. Protoc. 7(5), 946-958 (2012).

    2. Cochemé, H.M., Quin, C., McQuaker, S.J., et al. Measurement of H2O2 within living Drosophila during aging using a ratiometric mass spectrometry probe targeted to the mitochondrial matrix. Cell Metab. 13(3), 340-350 (2011).