An internal standard for the quantification of MitoB
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Unlabeled Version(s)
17116MitoB
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MitoB-d15

Item No. 17470

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
[(3-boronophenyl)methyl]triphenyl-d15-phosphonium, monobromide
Molecular Formula
C25H8D15BO2P • Br
Formula Weight
492.2
Purity
≥99% deuterated forms (d1-d15)
Formulation
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 5 mg/mlDMSO: 10 mg/mlDMSO:PBS (pH 7.2) (1:1): 0.5 mg/mlEthanol: 0.5 mg/ml
λmax
267 nm
SMILES
OB(O)C1=CC=CC(C[P+](C2=C([2H])C([2H])=C([2H])C([2H])=C2[2H])(C3=C([2H])C([2H])=C([2H])C([2H])=C3[2H])C4=C([2H])C([2H])=C([2H])C([2H])=C4[2H])=C1.[Br-]
InChi Code
InChI=1S/C25H23BO2P.BrH/c27-26(28)22-12-10-11-21(19-22)20-29(23-13-4-1-5-14-23,24-15-6-2-7-16-24)25-17-8-3-9-18-25;/h1-19,27-28H,20H2;1H/q+1;/p-1/i1D,2D,3D,4D,5D,6D,7D,8D,9D,13D,14D,15D,16D,17D,18D;
InChi Key
VDNRROALFUZCBO-CORGUAOTSA-M
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    1 mg

    $75

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    5 mg

    $333

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    10 mg

    $589

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    Product Description

    MitoB-d15 is intended for use as an internal standard for the quantification of MitoB (Item No. 17116) by GC- or LC-MS. MitoB is a ratiometric mass spectrometry probe that can be used for assessing changes in H2O2 within mitochondria in vivo. MitoB contains a triphenylphosphonium cation component that drives its accumulation in mitochondria where its arylboronic moiety selectively reacts with H2O2 to produce a phenol product, MitoP (Item No. 17117).1,2 Quantifying the MitoP/MitoB ratio by LC-MS/MS reflects the mitochondrial matrix H2O2 concentration.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Cochemé, H.M., Logan, A., Prime, T.A., et al. Using the mitochondria-targeted ratiometric mass spectrometry probe MitoB to measure H2O2 in living Drosophila. Nat. Protoc. 7(5), 946-958 (2012).

    2. Cochemé, H.M., Quin, C., McQuaker, S.J., et al. Measurement of H2O2 within living Drosophila during aging using a ratiometric mass spectrometry probe targeted to the mitochondrial matrix. Cell Metab. 13(3), 340-350 (2011).