A colorimetric peroxidase substrate
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o-Dianisidine (hydrochloride)

Item No. 17316

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
3,3'-dimethoxy-[1,1'-biphenyl]-4,4'-diamine, dihydrochloride
CAS Number
20325-40-0
Molecular Formula
C14H16N2O2 • 2HCl
Formula Weight
317.2
Purity
≥98%
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
PBS (pH 7.2): 5 mg/ml
λmax
210, 297 nm
SMILES
COC1=C(N)C=CC(C2=CC=C(N)C(OC)=C2)=C1.Cl.Cl
InChi Code
InChI=1S/C14H16N2O2.2ClH/c1-17-13-7-9(3-5-11(13)15)10-4-6-12(16)14(8-10)18-2;;/h3-8H,15-16H2,1-2H3;2*1H
InChi Key
UXTIAFYTYOEQHV-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    5 g

    $49

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    10 g

    $93

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    25 g

    $213

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    100 g

    $655

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    Product Description

    o-Dianisidine (dihydrochloride) is a colorimetric peroxidase substrate that has been used as a substrate to semi-quantitatively measure lactate, uric acid (Item No. 16219), and glucose.1,2 It has also been used as a reagent for the detection of various metals, thiocyanates, and nitrites.3 This aromatic amine is initially colorless but turns to violet upon oxidization and is spectrophotometrically measured at a wavelength of 405 nm.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Sugiura, M., and Hirano, K. A new colorimetric method for determination of serum glucose. Clin. Chim. Acta 75(3), 387-391 (1977).

    2. Mesulam, M.M., and Rosene, D.L. Sensitivity in horseradish peroxidase neurohistochemistry: A comparative and quantitative study of nine methods. J. Histochem. Cytochem. 27(3), 763-773 (1979).

    3. Program, N.T. 3,3'-Dimethoxybenzidine and dyes metabolized to 3,3'-dimethoxybenzidine. Rep. Carcinog. 12, 164-165 (2011).