A ferroptosis inhibitor
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Ferrostatin-1

Item No. 17729

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
3-amino-4-(cyclohexylamino)-benzoic acid, ethyl ester
CAS Number
347174-05-4
Synonyms
  • Fer-1
Molecular Formula
C15H22N2O2
Formula Weight
262.4
Purity
≥98%
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 30 mg/mlDMF:PBS (pH 7.2) (1:4): 0.2 mg/mlDMSO: 10 mg/mlEthanol: 10 mg/ml
λmax
217, 245, 323 nm
SMILES
NC(C=C(C(OCC)=O)C=C1)=C1NC2CCCCC2
InChi Code
InChI=1S/C15H22N2O2/c1-2-19-15(18)11-8-9-14(13(16)10-11)17-12-6-4-3-5-7-12/h8-10,12,17H,2-7,16H2,1H3
InChi Key
UJHBVMHOBZBWMX-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    5 mg

    $61

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    10 mg

    $109

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    25 mg

    $213

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    Product Description

    Ferrostatin-1 is a ferroptosis inhibitor.1 It inhibits cell death induced by the ferroptosis inducers erastin (Item No. 17754; EC50 = 60 nM) and RSL3, but not by hydrogen peroxide, rotenone, or staurosporine, in HT-1080 cells when used at a concentration of 0.5 μM. Ferrostatin-1 (0.5 μM) inhibits erastin-induced production of reactive oxygen species (ROS) and lipid peroxidation in HT-1080 cells. It increases cell survival in cell-based models of Huntington's disease, periventricular leukomalacia, and kidney proximal tubule damage in a concentration-dependent manner.2 Ferrostatin-1 (2 μM) inhibits cell death induced by L-glutamate in a rat organotypic hippocampal slice culture (OHSC) model.1 It also inhibits autooxidation of lipids by trapping peroxyl radicals.1,3

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Dixon, S.J., Lemberg, K.M., Lamprecht, M.R., et al. Ferroptosis: An iron-dependent form of non-apoptotic cell death. Cell 149(5), 1060-1072 (2012).

    2. Skouta, R., Dixon, S.J., Wang, J., et al. Ferrostatins inhibit oxidative lipid damage and cell death in diverse disease models. J. Am. Chem. Soc. 136(12), 4551-4556 (2014).

    3. Zilka, O., Shah, R., Li, B., et al. On the mechanism of cytoprotection by ferrostatin-1 and liproxstatin-1 and the role of lipid peroxidation in ferroptotic cell death. ACS Cent. Sci. 3(3), 232-243 (2017).

    Product Citations

    Zhang, L.J., Salekeen, R., Soto-Palma, C., et al. Article polyunsaturated lipid senolytics exploit a ferroptotic vulnerability in senescent cells. Cell Press Blue 1(1), 100004 (2026).

    Amoscato, A.A., Anthonymuthu, T., Kapralov, O., et al. Formation of protein adducts with hydroperoxy-PE electrophilic cleavage products during ferroptosis. Redox Biol. 63, 102758 (2023).

    Wang, M.-E., Chen, J., Lu, Y., et al. RB1-deficient prostate tumor growth and metastasis are vulnerable to ferroptosis induction via the E2F/ACSL4 axis. J. Clin. Invest. 133(10), e166647 (2023).

    Yoshioka, H., Kawamura, T., Muroi, M., et al. Identification of a small molecule that enhances ferroptosis via inhibition of ferroptosis suppressor protein 1 (FSP1). ACS Chem Biol. 17(2), 483-491 (2022).

    Hendricks, J.M., Doubravsky, C., Wehri, E., et al. Identification of structurally diverse FSP1 inhibitors that sensitize cancer cells to ferroptosis. Cell Chem. Biol. 30(9), P1090-P1103 (2022).

    ­Wang, F., Graham, E.T., Naowarojna, N., et al. PALP: A rapid imaging technique for stratifying ferroptosis sensitivity in normal and tumor tissues in situ. Cell Chem. Bio. 29, 157-170 (2022).

    Li, Z., Ferguson, L., Deol, K.K., et al. Ribosome stalling during selenoprotein translation exposes a ferroptosis vulnerability. Nat. Chem. Biol. (2022).

    Mishima, E., Ito, J., Wu, Z., et al. A non-canonical vitamin K cycle is a potent ferroptosis suppressor. Nature 608(7924), 778-783 (2022).

    Beatty, A., Singh, T., Tyurina, Y.Y., et al. Ferroptotic cell death triggered by conjugated linolenic acids is mediated by ACSL1. Nat. Commun. 12(1), 2244 (2021).

    Kobayashi, S., Harada, Y., Homma, T., et al. Characterization of a rat monoclonal antibody raised against ferroptotic cells. J. Immunol. Methods 489, 112912 (2021).

    Nagakannan, P., Islam, M.I., Conrad, M., et al. Cathepsin B is an excutioner of ferroptosis. Biochim. Biophys. Acta Mol. Cell Res. 1868(3), 118928 (2021).

    Soriano-Castell, D., Currais, A., and Maher, P. Defining a pharmacological inhibitor fingerprint for oxytosis/ferroptosis. Free Radic. Biol. Med. S0891-5849(21), (2021).

    Lien, T.S., Sun, D.-S., Hung, S.-C., et al. Dengue virus envelope protein domain III induces Nlrp3 inflammasome-dependent NETosis-mediated inflammation in mice. Front. Immunol. 12, 618577 (2021).

    Nakajima, W., Miyazaki, K., Asano, Y., et al. Krüppel-like factor 4 and its activator APTO-253 induce NOXA-mediated, p53-independent apoptosis in triple-negative breast cancer cells. Genes (Basel) 12(4), 539 (2021).

    Kremer, D.M., Nelson, B.S., Lin, L., et al. GOT1 inhibition primes pancreatic cancer for ferroptosis through the autophagic release of labile iron. bioRxiv (2020).

    Hu, S., Sechi, M., Singh, P.K., et al. A novel redox modulator induces a GPX4-mediated cell death that is dependent on iron and reactive oxygen species. J. Med. Chem. 63(17), 9838-9855 (2020).

    Bersuker, K., Hendricks, J., Li, Z., et al. The CoQ oxidoreductase FSP1 acts parallel to GPX4 to inhibit ferroptosis. Nature 575(7784), 688-692 (2019).

    Wang, W., Green, M., Choi, J.E., et al. CD8+ T cells regulate tumour ferroptosis during cancer immunotherapy. Nature 569(7755), 270-274 (2019).