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Discover high-quality research tools to investigate GLP-1 mechanisms and next-generation metabolic targets.
OBESITY RESEARCH SOLUTIONSThe main analytical pitfalls in insulin measurement in serum are related to (a) hemolysis, (b) circulating anti-insulin autoantibodies, and, for the past few years, (c) the reactivity (or lack of reactivity) of rapid- or long-acting pharmacological insulin analogs in sera.1 Hemolyzed samples contain an insulin-degrading enzyme and should not be analyzed unless they can be handled at 4°C within 2 to 3 hours or an insulinase inhibitor cocktail has been added in the blood collection tube to prevent insulin degradation. The Insulinase Inhibitor Cocktail shall have no impact on the assay robustness, and therefore have been validated with the analytical method used. This Inhibitor cocktail has been developed and validated with Bertin Pharma Mouse/Rat Insulin EIA kit (#A05105). [Bertin Catalog No. D05011]
WARNING This product is not for human or veterinary use.
1. Effect of hemolysis on the concentration of insulin in serum determined by RIA and IRMA. Clin. Chem. 44, 354-356 (2015).