SPM D-series MaxSpec^® LC-MS Mixture

This mixture contains several D-series resolvins (specialized pro-resolving mediators; SPMs) and their precursor, docosahexaenoic acid (DHA; Item No. 90310). The mixture is supplied in an amber ampule in which the headspace has been purged with argon to prevent lipid oxidation. This product has been designed for direct use in LC-MS applications. The solution may be serially diluted for preparation of calibrators and QC standards and/or used directly as a system suitability standard or tuning standard. After opening, we recommend that the mixture be transferred immediately to a 1 ml glass screw cap vial, to prevent solvent evaporation, and stored at -20°C. The mixture should be discarded after multiple freeze/thaw cycles.

The fatty acids in this mixture represent the metabolic cascade of the D-series resolvins, a family of lipid mediators that promote the resolution of the inflammatory response back to a non-inflamed state.¹ D-Series resolvins are produced physiologically from the sequential oxygenation of DHA by 15- and 5-lipoxygenase.² 17(R)-Resolvin D1 (Item No. 13060) is an aspirin-triggered epimer of resolvin D1 (Item No. 10012554) that, unlike resolvin D1, resists rapid inactivation by eicosanoid oxidoreductases.³

WARNING This product is not for human or veterinary use.

1. Ariel, A., and Serhan, C.N. Resolvins and protectins in the termination program of acute inflammation. Trends Immunol. 28(4), 176-183 (2007).

2. Hong, S., Gronert, K., Devchand, P.R., et al. Novel docosatrienes and 17S-resolvins generated from docosahexaenoic acid in murine brain, human blood, and glial cells. Autacoids in anti-inflammation. The Journal of Biological Chemisty 278(17), 14677-14687 (2003).

3. Sun, Y.P., Oh, S.F., Uddin, J., et al. Resolvin D1 and its aspirin-triggered 17R epimer stereochemical assignments, anti-inflammatory properties, and enzymatic inactivation. The Journal of Biological Chemisty 282(13), 9323-9334 (2007).