A cationic dye
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Rhodamine 110 (chloride)

Item No. 19061

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
3,6-diamino-9-(2-carboxyphenyl)-xanthylium, monochloride
CAS Number
13558-31-1
Synonyms
  • RH110
  • Rhodamine 560
  • Rhodamine N
Molecular Formula
C20H15N2O3 • Cl
Formula Weight
366.8
Purity
≥97%
Emission
496 nm
Excitation
496 nm
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: SolubleDMSO: SolubleEthanol: SolubleMethanol: SolubleWater: Slightly Soluble
λmax
246, 509 nm
SMILES
NC1=CC2=[O+]C3=C(C(C4=CC=CC=C4C(O)=O)=C2C=C1)C=CC(N)=C3.[Cl-]
InChi Code
InChI=1S/C20H14N2O3.ClH/c21-11-5-7-15-17(9-11)25-18-10-12(22)6-8-16(18)19(15)13-3-1-2-4-14(13)20(23)24;/h1-10H,21-22H2;1H
InChi Key
MYIOYATURDILJN-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    250 mg

    $79

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    1 g

    $246

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    Product Description

    Rhodamine 110 is a green fluorescent cationic dye with excitation and emission maxima of 496 and 520 nm, respectively.1 When incorporated with a hydrolytic substrate (e.g., proteinase or peptidase substrates), it can be used as a highly sensitive detection reagent in fluorescence-based enzyme assays.2 Rhodamine 110 has also been used in a fluorescence quenching method for determining trace nitrite and as a probe for cytochrome P450 activity.3,4

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Thatte, H.S., Rhee, J.-H., Zagarins, S.E., et al. Acidosis-induced apoptosis in human and porcine heart. Ann. Thorac. Surg. 77(4), 1376-1383 (2004).

    2. Hug, H., Los, M., Hirt, W., et al. Rhodamine 110-linked amino acids and peptides as substrates to measure caspase activity upon apoptosis induction in intact cells. Biochemistry 38(42), 13906-13911 (1999).

    3. Zhang, X., Wang, H., Fu, N.N., et al. A fluorescence quenching method for the determination of nitrite with Rhodamine 110. Spectrochimica Acta.A.Mol.Biomol.Spectrosc. 59(8), 1667-1772 (2003).

    4. Yatzeck, M.M., Lavis, L.D., Chao, T.Y., et al. A highly sensitive fluorogenic probe for cytochrome P450 activity in live cells. Bioorg. Med. Chem. Lett. 18(22), 5864-5866 (2008).