An inhibitor of apoptosome formation and caspase activation
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NS 3694

Item No. 19190

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
4-chloro-2-[[[[3-(trifluoromethyl)phenyl]amino]carbonyl]amino]-benzoic acid
CAS Number
426834-38-0
Synonyms
  • Apoptosis Inhibitor II
Molecular Formula
C15H10ClF3N2O3
Formula Weight
358.7
Purity
≥98%
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 25 mg/mlDMF:PBS (pH 7.2) (1:1): 0.5 mg/mlDMSO: 20 mg/mlEthanol: 14 mg/ml
λmax
224, 243, 269 nm
SMILES
ClC1=CC=C(C(O)=O)C(NC(NC2=CC=CC(C(F)(F)F)=C2)=O)=C1
InChi Code
InChI=1S/C15H10ClF3N2O3/c16-9-4-5-11(13(22)23)12(7-9)21-14(24)20-10-3-1-2-8(6-10)15(17,18)19/h1-7H,(H,22,23)(H2,20,21,24)
InChi Key
GNCZTZCPXFDPLI-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    5 mg

    $72

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    10 mg

    $134

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    25 mg

    $315

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    50 mg

    $523

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    Product Description

    Cytochrome c, which leaks from the mitochondrial intermembrane space into the cytosol, causes oligomerization of apoptotic protease-activating factor 1 (Apaf-1), forming a caspase activation complex known as the apoptosome.1 NS 3694 is a cell-permeable diarylurea compound that inhibits apoptosome formation and caspase activation.2 It blocks cytochrome c-induced caspase activation in HeLa cell cytosolic extracts with an IC50 value of approximately 50 µM.2 NS 3694 prevents the formation of apoptosomes, which prevents the association of procaspases with Apaf-1.2 It suppresses caspase activation and apoptosis in cells treated with either TNF-α or staurosporine (Item No. 81590).2 NS 3694 is used to examine the role of apoptosome-mediated caspase activation in cell death.3,4,5

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Nikoletopoulou, V., Markaki, M., Palikaras, K., et al. Crosstalk between apoptosis, necrosis and autophagy. Biochim. Biophys. Acta 1833(12), 3448-3459 (2013).

    2. Lademann, U., Cain, K., Gyrd-Hansen, M., et al. Diarylurea compounds inhibit caspase activation by preventing the formation of the active 700-kilodalton apoptosome complex. Mol. Cell. Biol. 23(21), 7829-7837 (2003).

    3. Zhao, C.Q., Zhang, Y.H., Jiang, S.D., et al. Both endoplasmic reticulum and mitochondria are involved in disc cell apoptosis and intervertebral disc degeneration in rats. Age (Dordr) 32(2), 161-177 (2010).

    4. Miyake, K., Bekisz, J., Zhao, T., et al. Apoptosis-inducing factor (AIF) is targeted in IFN-α2a-induced Bid-mediated apoptosis through Bak activation in ovarian cancer cells. Biochim. Biophys. Acta 1823(8), 1378-1388 (2012).

    5. Gauron, C., Rampon, C., Bouzaffour, M., et al. Sustained production of ROS triggers compensatory proliferation and is required for regeneration to proceed. Sci. Rep. 3:2084, (2013).