A potent inhibitor of protein kinase C
Technical Support & Resources

Information provided in the product description is from published literature. Due to the nature of scientific experimentation, your results (e.g., selectivity and effective concentrations) or specific application for this product may differ. If you have questions about how this product fits your application, please contact our technical support staff.

Visit our FAQ

Contact Us

Toll Free Phone (USA and Canada Only): (888) 526-5351
Direct Phone: (734) 975-3888

Request Technical Support

Technical Support Request

To streamline the process attach the appropriate questionnaire to your inquiry.

Download IHC QuestionnaireDownload WB Questionnaire

View Our Privacy Statement for details on how we use and protect your data. In addition, this site is protected by hCaptcha and its Privacy Policy and Terms of Service apply.

Staurosporine

Item No. 81590

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
2,3,10,11,12,13-hexahydro-10R-methoxy-9S-methyl-11R-methylamino-9S,13R-epoxy-1H,9H-diindolo[1,2,3-gh;3’,2’,1’-lm]pyrrolo[3,4-j][1,7]benzodiazonin-1-one
CAS Number
62996-74-1
Synonyms
  • Stsp
Molecular Formula
C28H26N4O3
Formula Weight
466.5
Purity
≥98%
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMSO: 500 µg/mlMethanol: 2 mg/ml
λmax
241, 295, 337, 355, 373 nm
SMILES
[H][C@]12N(C3=C(N([C@](O2)(C)[C@H](OC)[C@H](NC)C1)C4=C5C=CC=C4)C5=C(CNC6=O)C6=C37)C8=C7C=CC=C8
InChi Code
InChI=1S/C28H26N4O3/c1-28-26(34-3)17(29-2)12-20(35-28)31-18-10-6-4-8-14(18)22-23-16(13-30-27(23)33)21-15-9-5-7-11-19(15)32(28)25(21)24(22)31/h4-11,17,20,26,29H,12-13H2,1-3H3,(H,30,33)/t17-,20-,26-,28+/m1/s1
InChi Key
HKSZLNNOFSGOKW-FYTWVXJKSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
Recommended Products

Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

Download most recent Certificate of Analysis for this product

    500 µg

    $39

    Add

    1 mg

    $70

    Add

    5 mg

    $154

    Add

    10 mg

    $270

    Add
    Kinase Resource Center
    Discover Products & Resources for Kinase Research
    • Kinase inhibitors, screening libraries, assay kits, & more
    • Tools to study kinase signaling pathways:
      • Growth factor signaling
      • PI3K/Akt/mTOR
      • MAPKs (ERK, p38, & JNK)
      • JAK/STAT signaling
    • Articles, resources, & advice
    EXPLORE NOW
    Product Description

    Staurosporine (Stsp) is potent inhibitor of protein kinase C (PKC) from rat brain, exhibiting an IC50 value of 2.7 nM.1 It inhibits rat recombinant PKC-α approximately 100- and 1,000-fold better than PKC-δ and PKC-ζ, respectively.2 However, Stsp is non-selective in that it also inhibits the activity of a variety of other protein kinases, not only PKC isoforms.3 The biological effects of Stsp include cytotoxicity, relaxation of smooth muscle, and regulation of eNOS gene expression.3,4

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Tamaoki, T., Nomoto, H., Takahashi, I., et al. Staurosporine, a potent inhibitor of phospholipid/Ca++ dependent protein kinase. Biochem. Biophys. Res. Commun. 135, 397-402 (1986).

    2. McGlynn, E., Liβz, J., Reutener, S., et al. Expression and partial characterization of rat protein kinase C-δ and protein kinase C-ζ in insect cells using recombinant baculovirus. J. Cell. Biochem. 49, 239-250 (1992).

    3. Ruegg, U.T., and Burgess, G.M. Staurosporine, K-252 and UCN-01: Potent but nonspecific inhibitors of protein kinases. Trends Pharmacol. Sci. 10, 218-220 (1989).

    4. Li, H., and Förstermann, U. Structure-activity relationship of staurosporine analogs in regulating expression of endothelial nitric-oxide synthase gene. Mol. Pharmacol. 57, 427-435 (1999).

    Product Citations

    Lee, K.P., Elster, S., Epstein, B., et al. PD-1 protects expanding human T cells from premature restimulation-induced cell death by modulating TCR and CD28 signaling. Cell Death Dis. 17(1), 272 (2026).

    Nakamura, T., Hipp, C., Mourão, A.S.D., et al. Phase separation of FSP1 promotes ferroptosis. Nature 619, 371-377 (2023).

    Liu, X., Nie, L., Zhang, Y., et al. Actin cytoskeleton vulnerability to disulfide stress mediates disulfidptosis. Nat. Cell. Biol. 25(3), 404-414 (2023).

    Njomen, E., Vanecek, A., Lansdell, T.A., et al. Small molecule 20S proteasome enhancer regulates MYC protein stability and exhibits antitumor activity in multiple myeloma. Biomedicines 10(5), 938 (2022).

    Mishima, E., Ito, J., Wu, Z., et al. A non-canonical vitamin K cycle is a potent ferroptosis suppressor. Nature 608(7924), 778-783 (2022).

    Alavi, M.V. Tau phosphorylation and OPA1 proteolysis are unrelated events: Implications for Alzheimer’s Disease. Biochim. Biophys. Acta Mol. Cell Res. 1868(12), 119116 (2021).

    Rana, P.S., Gibbons, B.A., Vereninov, A.A., et al. Calibration and characterization of intracellular Asante Potassium Green probes, APG-2 and APG-4. Anal. Biochem. 567, 8-13 (2019).

    Bosnakovski, D., Choi, S.H., Strasser, J.M., et al. High-throughput screening identifies inhibitors of DUX4-induced myoblast toxicity. Skelet. Muscle 4(4), (2014).

    Ayers, S., Graf, T.N., Adcock, A.F., et al. Resorcylic acid lactones with cytotoxic and NF-κB inhibitory activities and their structure-activity relationships. J. Nat. Prod. 74(5), 1126-1131 (2011).