A fluorescent CYP450 substrate
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7-ethoxy-4-Methylcoumarin

Item No. 19586

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
7-ethoxy-4-methyl-2H-1-benzopyran-2-one
CAS Number
87-05-8
Synonyms
  • 4-Methyl-7-Ethoxycoumarin
  • 4-Methylumbelliferone ethyl ether
  • NSC 60561
Molecular Formula
C12H12O3
Formula Weight
204.2
Purity
≥98%
Emission
377 nm (in methanol), 449 nm (in dealkylase)
Excitation
318 nm (in methanol), 360 nm (in dealkylase)
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 30 mg/mLDMF:PBS (pH 7.2) (1:8): 0.1 mg/mLDMSO: 25 mg/mLEthanol: 10 mg/mL
λmax
220, 321 nm
SMILES
O=C1C=C(C)C2=CC=C(OCC)C=C2O1
InChi Code
InChI=1S/C12H12O3/c1-3-14-9-4-5-10-8(2)6-12(13)15-11(10)7-9/h4-7H,3H2,1-2H3
InChi Key
NKRISXMDKXBVRJ-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    250 mg

    $129

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    500 mg

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    1 g

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    Product Description

    7-ethoxy-4-Methylcoumarin is a fluorophore that has been used to monitor functional activity of cytochrome P450 (CYP)1A1, CYP2B4, and CYP2B6.1,2 It demonstrates excitation/emission maxima of 318 and 377 nm, respectively in methanol and 360 and 449 nm, respectively in dealkylase.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Liu, J., Shah, M.B., Zhang, Q., et al. Coumarin derivatives as substrate probes of mammalian cytochromes P450 2B4 and 2B6: Assessing the importance of 7-alkoxy chain length, halogen substitution, and non-active site mutations. Biochem. 55(13), 1997-2007 (2016).

    2. Kuhn, U.D., Splinter, F.K., Rost, M., et al. Induction of cytochrome P450 1A1 in rat liver slices by 7-ethoxycoumarin and 4-methyl-7-ethoxycoumarin. Exp. Toxicol. Pathol. 50(4-6), 491-496 (1998).