For immunochemical detection of 8-hydroxy-2'-deoxyguanosine and 8-hydroxyguanosine
Technical Support & Resources

Visit our FAQ

Contact Us

Toll Free Phone (USA and Canada Only): (888) 526-5351
Direct Phone: (734) 975-3888

Request Technical Support

Technical Support Request

To streamline the process attach the appropriate questionnaire to your inquiry.

Download IHC QuestionnaireDownload WB Questionnaire

View Our Privacy Statement for details on how we use and protect your data. In addition, this site is protected by hCaptcha and its Privacy Policy and Terms of Service apply.

DNA/RNA Oxidative Damage Monoclonal Antibody (Clone 7E6)

Item No. 20094

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • 8-OH-dG
  • 8-OHG
  • 8-oxo-G
  • 7,8-dihydro-8-oxoguanosine
Immunogen
8-Hydroxy-2-deoxyguanosine
Clone Designation
7E6
Formulation (Request formulation change)
100 µg of ammonium sulfate purified IgG
Storage Buffer
PBS, pH 7.2, with 50% glycerol, 0.1% BSA, and 0.02% sodium azide
Host
Mouse
Isotype
IgG1
Applications
ELISA and IP
Cross Reactivity
(+) 8-hydroxy-2-deoxyguanosine(+) 8-hydroxy Guanosine
Species Reactivity
(Independent) Species
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
Recommended Products

Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

Download most recent Certificate of Analysis for this product

    100 µg

    $440

    Add
    Product Description

    8-Hydroxy-2'-deoxyguanosine (8-OH-dG; Item No. 89320) and 8-hydroxyguanosine (8-OHG; Item No. 89300) are oxidized nucleosides and markers of DNA and RNA oxidative damage, respectively.1,2 Levels of 8-OH-dG increase in DNA in the presence of eugenol and hydrogen peroxide or copper sulfate.3 Levels of 8-OHG increase in response to hydrogen peroxide in HeLa cells in a concentration-dependent manner.2 8-OHG has been detected in neurons in postmortem brain from patients with Alzheimer's or Parkinson's disease.4,5 Cayman's DNA/RNA Oxidative Damage Monoclonal Antibody (Clone 7E6) can be used for affinity purification and ELISA applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Kasai, H. Analysis of a form of oxidative DNA damage, 8-hydroxy-2'-deoxyguanosine, as a marker of cellular oxidative stress during carcinogenesis. Mutat. Res. 387(3), 147-163 (1997).

    2. Wu, J., and Li, Z. Human polynucleotide phosphorylase reduces oxidative RNA damage and protects HeLa cell against oxidative stress. Biochem. Biophys. Res. Commun. 372(2), 288-292 (2008).

    3. Bodell, W.J., Ye, Q., Pathak, D.N., et al. Oxidation of eugenol to form DNA adducts and 8-hydroxy-2'-deoxyguanosine: Role of quinone methide derivative in DNA adduct formation. Carcinogenesis 19(3), 437-443 (1998).

    4. Nunomura, A., Perry, G., Pappolla, M.A., et al. RNA oxidation is a prominent feature of vulnerable neurons in Alzheimer’s disease. J. Neurosci. 19(6), 1959-1964 (1999).

    5. Zhang, J., Perry, G., Smith, M.A., et al. Parkinson’s disease is associated with oxidative damage to cytoplasmic DNA and RNA in substantia nigra neurons. Am. J. Pathol. 154(5), 1423-1429 (1999).

    Product Citations

    Nunomura, A., Perry, G., Pappolla, M.A., et al. RNA oxidation is a prominent feature of vulnerable neurons in Alzheimer’s disease. J. Neurosci. 19(6), 1959-1964 (1999).