For immunochemical detection of MPO
Technical Support & Resources

Visit our FAQ

Contact Us

Toll Free Phone (USA and Canada Only): (888) 526-5351
Direct Phone: (734) 975-3888

Request Technical Support

Technical Support Request

To streamline the process attach the appropriate questionnaire to your inquiry.

Download IHC QuestionnaireDownload WB Questionnaire

View Our Privacy Statement for details on how we use and protect your data. In addition, this site is protected by hCaptcha and its Privacy Policy and Terms of Service apply.

Myeloperoxidase (mouse) Polyclonal Antibody

Item No. 20493

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • MPO
Immunogen
Full-length recombinant mouse MPO
Formulation (Request formulation change)
500 µl of protein A-purified polyclonal antibody
Storage Buffer
TBS, pH 7.4, with 50% glycerol, 0.1% BSA, and 0.02% sodium azide
Host
Rabbit
Applications
ELISA, IHC, WB
Species Reactivity
(+) Mouse; other species not tested
UniProt Accession №
P11247
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
Recommended Products

Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

Download most recent Certificate of Analysis for this product

    1 ea (500 µl)

    $440

    Add
    NET Formation Screening & Analysis Services
    Our experts are here to help.
    • Detection of NET formation ex vivo and screening for modulators using:
      • High-content imaging
      • Enzymatic detection
      • Citrullination/carbamylation detection
    • Experienced scientists skilled in neutrophil biology, isolation, and handling
    LEARN MORE
    Product Description

    Myeloperoxidase (MPO) is a heme-containing enzyme and the most abundant protein in polymorphonuclear leukocytes (PMNs).1 It is composed of two subunits linked by a disulfide bridge with each subunit containing a light and a heavy polypeptide chain. It can oxidize a variety of substrates and catalyzes the formation of highly reactive (pseudo)hypohalous acids and radicals including hypochlorous acid. MPO is stored in azurophilic granules of PMNs and is released from activated or necrotic PMNs, after which it can bind to and modify acidic serum proteins, as well as recruit additional PMNs. MPO also has roles in PMN apoptosis and antimicrobial defense systems, including neutrophil extracellular traps (NETs).1,2,3 MPO-deficient mice exhibit reduced survival in a polymicrobial sepsis model, increased susceptibility to experimental autoimmune encephalomyelitis (EAE), and increased atherosclerosis in mice also deficient in the LDL receptor and fed an atherogenic diet.1,4,5 Cayman's Myeloperoxidase (mouse) Polyclonal Antibody can be used for ELISA, immunohistochemistry (IHC), and Western blot (WB) applications. The antibody recognizes MPO at approximately 80 kDa from mouse samples.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Arnhold, J., and Flemmig, J. Human myeloperoxidase in innate and acquired immunity. Arch. Biochem. Biophys. 500(1), 92-106 (2010).

    2. Metzler, K.D., Fuchs, T.A., Nauseef, W.M., et al. Myeloperoxidase is required for neutrophil extracellular trap formation: Implications for innate immunity. Blood 117(3), 953-959 (2011).

    3. Urban, C.F., Ermert, D., Schmid, M., et al. Neutrophil extracellular traps contain calprotectin, a cytosolic protein complex involved in host defense against Candida albicans. PLoS Pathog. 5(10), e1000639 (2009).

    4. Brennan, M., Gaur, A., Pahuja, A., et al. Mice lacking myeloperoxidase are more susceptible to experimental autoimmune encephalomyelitis. J. Neuroimmunol. 112(1-2), 97-105 (2001).

    5. Brennan, M.L., Anderson, M.M., Shih, D.M., et al. Increased atherosclerosis in myeloperoxidase-deficient mice. J. Clin. Invest. 107(4), 419-430 (2001).