For immunochemical detection of MPO
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Myeloperoxidase (human) Rabbit Monoclonal Antibody (Clone RM407)

Item No. 32333

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • MPO
Immunogen
Peptide corresponding to the internal region of the human MPO light chain
Clone Designation
RM407
Formulation (Request formulation change)
100 µl of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
IHC, WB
Cross Reactivity
(+) MPO
Species Reactivity
(+) Human
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    100 µl

    $411

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    NET Formation Screening & Analysis Services
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    • Detection of NET formation ex vivo and screening for modulators using:
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    Product Description

    Myeloperoxidase (MPO) is a heme-containing enzyme and the most abundant protein in polymorphonuclear leukocytes (PMNs).1 It is composed of two subunits linked by a disulfide bridge with each subunit containing a light and a heavy polypeptide chain. It can oxidize a variety of substrates and catalyzes the formation of highly reactive (pseudo)hypohalous acids and radicals including hypochlorous acid. MPO is stored in azurophilic granules of PMNs and is released from activated or necrotic PMNs, after which it can bind to and modify acidic serum proteins, as well as recruit additional PMNs. MPO also has roles in PMN apoptosis and antimicrobial defense systems, including neutrophil extracellular traps (NETs).1,2,3 MPO-deficient mice exhibit reduced survival in a polymicrobial sepsis model, increased susceptibility to experimental autoimmune encephalomyelitis (EAE), and increased atherosclerosis in mice also deficient in the LDL receptor and fed an atherogenic diet.1,4,5 Cayman's Myeloperoxidase (human) Rabbit Monoclonal Antibody (Clone RM407) can be used for immunohistochemistry (IHC) and Western blot (WB) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Arnhold, J., and Flemmig, J. Human myeloperoxidase in innate and acquired immunity. Arch. Biochem. Biophys. 500(1), 92-106 (2010).

    2. Metzler, K.D., Fuchs, T.A., Nauseef, W.M., et al. Myeloperoxidase is required for neutrophil extracellular trap formation: Implications for innate immunity. Blood 117(3), 953-959 (2011).

    3. Urban, C.F., Ermert, D., Schmid, M., et al. Neutrophil extracellular traps contain calprotectin, a cytosolic protein complex involved in host defense against Candida albicans. PLoS Pathog. 5(10), e1000639 (2009).

    4. Brennan, M., Gaur, A., Pahuja, A., et al. Mice lacking myeloperoxidase are more susceptible to experimental autoimmune encephalomyelitis. J. Neuroimmunol. 112(1-2), 97-105 (2001).

    5. Brennan, M.L., Anderson, M.M., Shih, D.M., et al. Increased atherosclerosis in myeloperoxidase-deficient mice. J. Clin. Invest. 107(4), 419-430 (2001).