A fluorogenic dye
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Calcein Red™ AM

Item No. 20632

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Calcein Red™ Acetoxymethyl ester
Formula Weight
1015.8
Purity
≥90%
Emission
574 nm
Excitation
560 nm
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMSO: Soluble
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

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    1 mg

    $295

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    Product Description

    Calcein Red™ AM is a fluorogenic dye that is used to assess cell viability. As the acetoxymethyl ester, Calcein Red™ AM is non-fluorescent but cell-permeant and is hydrolyzed to the fluorescent probe Calcein Red™ (Item No. 20633) by intracellular esterases. Calcein Red™ is retained by living cells and displays excitation/emission maxima of 560/574 nm, respectively. Calcein Red™ AM is a red-emitting variant of the green fluorophore calcein AM (Item No. 14948), which has been used to evaluate cell viability and cytotoxicity.1,2

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Bratosin, D., Mitrofan, L., Palii, C., et al. Novel fluorescence assay using calcein-AM for the determination of human erythrocyte viability and aging. Cytometry 66(1), 78-84 (2005).

    2. Lévesque, A., Paquet, A., and Pagé, M. Measurement of tumor necrosis factor activity by flow cytometry. Cytometry 20(2), 181-184 (1995).

    Product Citations

    Bailey, C.P., Figueroa, M., Gangadharan, A., et al. Scaffolding LSD1 inhibitors impair NK cell metabolism and cytotoxic function through depletion of glutathione. Front. Immunol. 11, 2196 (2020).