A membrane-impermeant fluorescent dye
Technical Support & Resources

Information provided in the product description is from published literature. Due to the nature of scientific experimentation, your results (e.g., selectivity and effective concentrations) or specific application for this product may differ. If you have questions about how this product fits your application, please contact our technical support staff.

Visit our FAQ

Contact Us

Toll Free Phone (USA and Canada Only): (888) 526-5351
Direct Phone: (734) 975-3888

Request Technical Support

Technical Support Request

To streamline the process attach the appropriate questionnaire to your inquiry.

Download IHC QuestionnaireDownload WB Questionnaire

View Our Privacy Statement for details on how we use and protect your data. In addition, this site is protected by hCaptcha and its Privacy Policy and Terms of Service apply.

Product Categories

Product Type

Biochemicals
Labeling & Detection
Fluorescent Probes

Research Area

Application

Flow Cytometry
Fluorescence

Calcein Blue

Item No. 20636

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
N-(carboxymethyl)-N-[(7-hydroxy-4-methyl-2-oxo-2H-1-benzopyran-8-yl)methyl]-glycine
CAS Number
54375-47-2
Molecular Formula
C15H15NO7
Formula Weight
321.3
Purity
≥90%
Emission
445 nm
Excitation
360 nm
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMSO: soluble
λmax
322 nm
SMILES
OC1=CC=C(C(C)=CC(O2)=O)C2=C1CN(CC(O)=O)CC(O)=O
InChi Code
InChI=1S/C15H15NO7/c1-8-4-14(22)23-15-9(8)2-3-11(17)10(15)5-16(6-12(18)19)7-13(20)21/h2-4,17H,5-7H2,1H3,(H,18,19)(H,20,21)
InChi Key
RJPSHDMGSVVHFA-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
Recommended Products

Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

Download most recent Certificate of Analysis for this product

    25 mg

    $169

    Add
    Product Description

    Calcein Blue is a membrane-impermeant fluorescent dye. It is a blue-emitting variant of the green fluorophore Calcein (Item No. 16221), which has been entrapped in liposomes and similar vesicles for studying membrane characteristics.1,2 Calcein Blue displays excitation/emission maxima of 360/445 nm.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Sine, J., Urban, C., Thayer, D., et al. Photo activation of HPPH encapsulated in "Pocket" liposomes triggers multiple drug release and tumor cell killing in mouse breast cancer xenografts. Int. J. Nanomedicine 10, 125-145 (2014).

    2. Sudji, I.R., Subburaj, Y., Frenkel, N., et al. Membrane disintegration caused by the steroid saponin digitonin is related to the presence of cholesterol. Molecules 20(11), 20146-20160 (2015).