A fluorogenic dye
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Calcein UltraBlue™ AM

Item No. 20639

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Calcein UltraBlue™ Acetoxymethyl ester
Formula Weight
788.7
Purity
≥90%
Emission
445 nm
Excitation
360 nm
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMSO: soluble
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

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    50 µg

    $63

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    Product Description

    Calcein UltraBlue™ AM is a fluorogenic dye that is used to assess cell viability. As the acetoxymethyl ester, Calcein UltraBlue™ AM is non-fluorescent but cell-permeant and is hydrolyzed to the fluorescent probe Calcein UltraBlue™ (Item No. 20638) by intracellular esterases. Calcein UltraBlue™ is retained by living cells and displays excitation/emission maxima of 360/445 nm, respectively. Calcein UltraBlue™ AM is a dark blue-emitting variant of the green fluorophore calcein AM (Item No. 14948), which has been used to evaluate cell viability and cytotoxicity.1,2

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Bratosin, D., Mitrofan, L., Palii, C., et al. Novel fluorescence assay using calcein-AM for the determination of human erythrocyte viability and aging. Cytometry 66(1), 78-84 (2005).

    2. Lévesque, A., Paquet, A., and Pagé, M. Measurement of tumor necrosis factor activity by flow cytometry. Cytometry 20(2), 181-184 (1995).