For immunochemical detection of H3S10Ph
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Histone H3S10Ph Monoclonal Antibody (Clone RM163)

Item No. 20720

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • H3pS10
  • H3pSer10
  • Histone H3 (Phospho-Ser10)
  • Phospho-Histone H3 Serine 10
  • Phosphorylated Histone H3 Serine 10
Immunogen
Peptide corresponding to H3S10Ph
Clone Designation
RM163
Formulation (Request formulation change)
100 µg of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
ELISA, ICC, multiplex-based assay, WB
Cross Reactivity
(+) H3S10Ph(-) Other phosphorylated histones
Species Reactivity
(+) Vertebrates
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    100 µg

    $500

    Add
    Cayman Chemical
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    Product Description

    Histone H3 is a nuclear protein and a component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei.1 It is a globular protein that contains an unstructured N-terminal tail that extends outside of the nucleosome core and is subject to various post-translational modifications (PTMs), including methylation, phosphorylation, acetylation, and citrullination.1,2 Phosphorylation of histone H3 at serine 10 (H3S10Ph) is correlated with chromatin condensation during mitosis and with transcriptional activation of genes during interphase.3,4 H3S10Ph does not inhibit binding of the ADD domain of the chromatin-remodeling protein ATRX to histone H3 N-terminal peptides due to its positioning away from the core peptide binding sequence but does decrease binding of the HP1α chromodomain (CD) when the histone H3 peptide is also trimethylated at lysine 9.5 Low median levels of H3S10Ph in tumor tissue isolated from patients with oral squamous cell carcinoma (OSCC) are associated with cervical lymph node metastasis.6 Cayman's Histone H3S10Ph Monoclonal Antibody (Clone RM163) can be used for ELISA, immunocytochemistry (ICC), multiplex-based assay, and Western blot (WB) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Hyun, K., Jeon, J., Park, K., et al. Writing, erasing and reading histone lysine methylations. Exp. Mol. Med. 49(4), e324 (2017).

    2. Sharda, A., Amnekar, R.V., Natu, A., et al. Histone posttranslational modifications: Potential role in diagnosis, prognosis, and therapeutics of cancer. Prognostic Epigenetics 15, 351-373 (2019).

    3. Sawicka, A., and Seiser, C. Histone H3 phosphorylation - A versatile chromatin modification for different occasions. Biochimie 94(11), 2193-2201 (2012).

    4. Prigent, C., and Dimitrov, S. Phosphorylation of serine 10 in histone H3, what for? J. Cell. Sci. 116(Pt. 18), 3677-3685 (2003).

    5. Noh, K.M., Maze, I., Zhao, D., et al. ATRX tolerates activity-dependent histone H3 methyl/phos switching to maintain repetitive element silencing in neurons. Proc. Natl. Acad. Sci. USA 112(22), 6820-6827 (2015).

    6. Campos-Fernández, E., Matsuo, F.S., Andrade, M.F., et al. Prognostic value of histone H3 serine 10 phosphorylation and histone H4 lysine 12 acetylation in oral squamous cell carcinoma. Histopathology 74(2), 227-238 (2019).