A red fluorescent calcium indicator
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Alternative(s)
20778Rhod-2 (sodium salt)
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Rhod-2 (potassium salt)

Item No. 20777

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
2,2'-((2-(2-(2-(bis(carboxylatomethyl)amino)-5-(6-(dimethylamino)-3-(dimethyliminio)-3H-xanthen-9-yl)phenoxy)ethoxy)-4-methylphenyl)azanediyl)diacetate, tripotassium salt
CAS Number
663625-18-1
Molecular Formula
C40H39N4O11 • 3K
Formula Weight
869.1
Purity
≥90%
Emission
581 nm (Ca2+-bound)
Excitation
549 nm (Ca2+-free), 552 nm (Ca2+-bound)
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMSO: solubleWater: soluble
SMILES
CN(C)C(C=C1)=CC2=C1C(C3=CC=C(N(CC([O-])=O)CC([O-])=O)C(OCCOC4=C(N(CC([O-])=O)CC([O-])=O)C=CC(C)=C4)=C3)=C(C=C/5)C(O2)=CC5=[N+](C)\C.[K+].[K+].[K+]
InChi Code
InChI=1S/C40H42N4O11.3K/c1-24-6-12-30(43(20-36(45)46)21-37(47)48)34(16-24)53-14-15-54-35-17-25(7-13-31(35)44(22-38(49)50)23-39(51)52)40-28-10-8-26(41(2)3)18-32(28)55-33-19-27(42(4)5)9-11-29(33)40;;;/h6-13,16-19H,14-15,20-23H2,1-5H3,(H3-,45,46,47,48,4
InChi Key
ZFYWHSXZMYZBNV-UHFFFAOYSA-K
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    1 mg

    $303

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    Product Description

    Rhod-2 (potassium salt) is a water-soluble, red fluorescent calcium indicator. It exhibits a significant shift in fluorescence intensity upon calcium binding (ex max = 549 nm; calcium-free v. ex/em max = 552/581 nm; calcium-bound).1,2 Unlike the UV-excitable indicators fura-2 (Item Nos. 19531 | 20414) and indo-1 (Item Nos. 20419 | 20418), there is no accompanying spectral shift.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Paredes, R.M., Etzler, J.C., Watts, L.T., et al. Chemical calcium indicators. Methods 46(3), 143-151 (2008).

    2. Minta, A., Kao, J.P., and Tsien, R.Y. Fluorescent indicators for cytosolic calcium based on rhodamine and fluorescein chromophores. The Journal of Biological Chemisty 264(14), 8171-8178 (1989).