A demethylated variant of a RIP1 kinase inhibitor
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Necrostatin-1 Inactive Control

Item No. 21192

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
5-(1H-indol-3-ylmethyl)-2-thioxo-4-imidazolidinone
CAS Number
64419-92-7
Synonyms
  • Nec-1i
  • Nec-1 Inactive Control​
Molecular Formula
C12H11N3OS
Formula Weight
245.3
Purity
≥98%
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 20 mg/mlDMF:PBS (pH 7.2) (1:1): 0.5 mg/mlDMSO: 10 mg/mlEthanol: 1 mg/ml
λmax
220, 267 nm
SMILES
O=C(C(N1)CC2=CNC3=CC=CC=C32)NC1=S
InChi Code
InChI=1S/C12H11N3OS/c16-11-10(14-12(17)15-11)5-7-6-13-9-4-2-1-3-8(7)9/h1-4,6,10,13H,5H2,(H2,14,15,16,17)
InChi Key
MPLRRPKKFHUEEL-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

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    1 mg

    $38

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    5 mg

    $129

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    10 mg

    $238

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    50 mg

    $1,083

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    Product Description

    Necrostatin-1 Inactive Control (Nec-1i) is a demethylated variant of necrostatin-1 (Nec-1; Item No. 11658), a RIP1 kinase inhibitor.1 While both Nec-1 and Nec-1i inhibit the immune regulator indoleamine 2,3-dioxygenase, Nec1i is ~100-fold less effective than Nec-1 in inhibiting RIP1 kinase in vitro and 10-fold less potent than Nec-1 in a mouse necroptosis assay.2 However, equally high doses of Nec-1 and Nec-1i are reported to inhibit TNF-induced systemic inflammatory response syndrome in vivo.2 Nec-1i is often used as an inactive control in studies using Nec-1 to exclude nonspecific off-target effects.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Degterev, A., Hitomi, J., Germscheid, M., et al. Identification of RIP1 kinase as a specific cellular target of necrostatins. Nat. Chem. Biol. 4(5), 313-321 (2008).

    2. Takahashi, N., Duprez, L., Grootjans, S., et al. Necrostatin-1 analogues: Critical issues on the specificity, activity and in vivo use in experimental disease models. Cell Death Dis. 3(11), e437 (2012).