A substrate for DPP IV
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Gly-Pro-pNA (hydrochloride)

Item No. 21244

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
glycyl-N-(4-nitrophenyl)-L-prolinamide, monohydrochloride
CAS Number
103213-34-9
Synonyms
  • Gly-Pro p-nitroanilide
  • GP-pNA
Molecular Formula
C13H16N4O4 • HCl
Formula Weight
328.8
Purity
≥98%
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 30 mg/mlDMSO: 30 mg/mlEthanol: 30 mg/mlPBS (pH 7.2): 10 mg/ml
λmax
221, 312 nm
SMILES
O=C(CN)N1CCC[C@H]1C(NC2=CC=C([N+]([O-])=O)C=C2)=O.Cl
InChi Code
InChI=1S/C13H16N4O4.ClH/c14-8-12(18)16-7-1-2-11(16)13(19)15-9-3-5-10(6-4-9)17(20)21;/h3-6,11H,1-2,7-8,14H2,(H,15,19);1H/t11-;/m0./s1
InChi Key
VBBFIBMVFSUUBP-MERQFXBCSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    10 mg

    $43

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    25 mg

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    50 mg

    $172

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    100 mg

    $321

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    Cayman Chemical
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    OBESITY RESEARCH SOLUTIONS
    Product Description

    Gly-Pro-pNA is a chromogenic substrate that can be cleaved by the circulating enzyme, dipeptidyl peptidase IV (DPP IV).1 Enzyme activity can be quantified by colorimetric detection of free p-nitroanilide at 405 nm. DPP IV inactivates the two peptides responsible for a majority of nutrient-stimulated insulin secretion: glucose-dependent insulinotropic polypeptide (GIP) and glucagon-like peptide 1 (GLP-1). This substrate can be used to screen for DPP IV inhibitors, which have emerged as a new class of experimental antidiabetic agents.2

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Kim, Y.B., Kopcho, L.M., Kirby, M.S., et al. Mechanism of Gly-Pro-pNA cleavage catalyzed by dipeptidyl peptidase-IV and its inhibition by saxagliptin (BMS-477118). Arch. Biochem. Biophys. 445(1), 9-18 (2006).

    2. Kushwaha, R.N., Haq, W., and Katti, S.B. Discovery of 17 gliptins in 17-years of research for the treatment of type 2 diabetes: A synthetic overview. Chem. Biol. Interface 4(3), 137-162 (2014).