An activator of AMPK
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PT1

Item No. 21335

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
2-chloro-5-[[5-[[5-(4,5-dimethyl-2-nitrophenyl)-2-furanyl]methylene]-4,5-dihydro-4-oxo-2-thiazolyl]amino]-benzoic acid
CAS Number
331002-70-1
Molecular Formula
C23H16ClN3O6S
Formula Weight
497.9
Purity
≥95%
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 1 mg/mlDMSO: 1 mg/mlDMSO:PBS(pH 7.2) (1:4): 0.2 mg/mlEthanol: slightly
λmax
272, 399 nm
SMILES
ClC1=CC=C(NC(S/C2=C/C3=CC=C(C4=CC(C)=C(C)C=C4[N+]([O-])=O)O3)=NC2=O)C=C1C(O)=O
InChi Code
InChI=1S/C23H16ClN3O6S/c1-11-7-16(18(27(31)32)8-12(11)2)19-6-4-14(33-19)10-20-21(28)26-23(34-20)25-13-3-5-17(24)15(9-13)22(29)30/h3-10H,1-2H3,(H,29,30)(H,25,26,28)/b20-10+
InChi Key
RTHRCOIONCZINZ-KEBDBYFISA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    1 mg

    $41

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    5 mg

    $128

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    10 mg

    $211

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    25 mg

    $487

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    Cayman Chemical
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    OBESITY RESEARCH SOLUTIONS
    Product Description

    PT1 is an activator of AMP-activated protein kinase (AMPK) that directly activates the inactive truncated forms of AMPK monomers α1335, α1394, and α2398 in a dose-dependent manner (EC50s = ~ 8, 8, and 12 µM, respectively).1 It stimulates the α1β1γ1 AMPK heterotrimer with an EC50 value of 0.3 μM.1 PT1 dose-dependently increases phosphorylation of AMPK and its downstream substrate, acetyl-CoA carboxylase, without increasing the cellular AMP:ATP ratio in L6 myotubes.1 In HepG2 liver cells, PT1 lowers lipid content through AMPK activation. PT1 induces autophagy in cardiomyocytes after oxygen glucose deprivation/reoxygenation, resulting in improved cell survival.2

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Pang, T., Zhang, Z.-S., Gu, M., et al. Small molecule antagonizes autoinhibition and activates AMP-activated protein kinase in cells. The Journal of Biological Chemisty 283(23), 16051-16060 (2008).

    2. Huang, L., Dai, K.H., Chen, M., et al. The AMPK agonist PT1 and mTOR inhibitor 3HOI-BA-01 protect cardiomyocytes after ischemia through induction of autophagy. J. Cardiovasc. Pharmacol. Ther. 21(1), 70-81 (2016).