A reagent that is used to alkylate thiophosphates
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Biochemicals

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Application

Immunoprecipitation
Western Blot

p-Nitrobenzyl mesylate

Item No. 21456

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
4-nitro-benzenemethanol, 1-methanesulfonate
CAS Number
39628-94-9
Synonyms
  • 4-Nitrobenzyl mesylate
  • para-Nitrobenzyl mesylate
  • PNBM
Molecular Formula
C8H9NO5S
Formula Weight
231.2
Purity
≥99%
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMSO: 100 mM
SMILES
CS(OCC1=CC=C([N+]([O-])=O)C=C1)(=O)=O
InChi Code
InChI=1S/C8H9NO5S/c1-15(12,13)14-6-7-2-4-8(5-3-7)9(10)11/h2-5H,6H2,1H3
InChi Key
QZIYKAFPHRTIAI-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    500 µg

    $49

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    1 mg

    $86

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    5 mg

    $329

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    Product Description

    p-Nitrobenzyl mesylate (PNBM) is a reagent that is used to alkylate thiophosphates, forming thiophosphate ester epitopes that can be recognized by specific antibodies.1 Substrates phosphorylated with adenosine 5'-(γ-thio)-triphosphate (ATPγS; Item No. 14957) or N6-benzyl-ATPγS can be alkylated by PNBM.2,3 The tagged substrates can be isolated by immunoprecipitation or immunoaffinity purification, or they can be detected by immunoblotting.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Allen, J.J., Li, M., Brinkworth, C.S., et al. A semisynthetic epitope for kinase substrates. Nat. Methods 4(6), 511-516 (2007).

    2. Kumar, V., Weng, Y.-C., Geldenhuys, W.J., et al. Generation and characterization of ATP analog-specific protein kinase Cδ. The Journal of Biological Chemisty 290(4), 1936-1951 (2015).

    3. Leissing, F., Nomoto, M., Bocola, M., et al. Substrate thiophosphorylation by Arabidopsis mitogen-activated protein kinases. BMC Plant Biol. 16(48), (2016).