A neuropeptide and agonist of NPFF1 and NPFF2 receptors
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Neuropeptide FF (trifluoroacetate salt)

Item No. 24548

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
L-phenylalanyl-L-leucyl-L-phenylalanyl-L-glutaminyl-L-prolyl-L-glutaminyl-L-arginyl-L-phenylalaninamide, trifluoroacetate salt
CAS Number
99566-27-5
Synonyms
  • NPFF
Molecular Formula
C54H76N14O10 • XCF3COOH
Formula Weight
1081.3
Purity
≥95%
Formulation (Request formulation change)
A lyophilized powder
Solubility(Learn about Variance in Solubility)
Water: 1 mg/ml
SMILES
NC([C@@H](NC([C@H](CCCNC(N)=N)NC([C@H](CCC(N)=O)NC([C@@H]1CCCN1C([C@H](CCC(N)=O)NC([C@@H](NC([C@H](CC(C)C)NC([C@@H](N[H])CC2=CC=CC=C2)=O)=O)CC3=CC=CC=C3)=O)=O)=O)=O)=O)CC4=CC=CC=C4)=O.FC(F)(C(O)=O)F
InChi Code
InChI=1S/C54H76N14O10.C2HF3O2/c1-32(2)28-41(66-47(72)36(55)29-33-14-6-3-7-15-33)50(75)67-42(31-35-18-10-5-11-19-35)51(76)64-39(23-25-45(57)70)53(78)68-27-13-21-43(68)52(77)63-38(22-24-44(56)69)49(74)62-37(20-12-26-61-54(59)60)48(73)65-40(46(58)71)30-34-16-8-4-9-17-34;3-2(4,5)1(6)7/h3-11,14-19,32,36-43H,12-13,20-31,55H2,1-2H3,(H2,56,69)(H2,57,70)(H2,58,71)(H,62,74)(H,63,77)(H,64,76)(H,65,73)(H,66,72)(H,67,75)(H4,59,60,61);(H,6,7)/t36-,37-,38-,39-,40-,41-,42-,43-;/m0./s1
InChi Key
MIXMSRWGDOFYHN-HBBGHHHDSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    1 mg

    $174

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    Product Description

    Neuropeptide FF (NPFF) is a peptide expressed in the brain and spinal cord that shares a precursor protein with neuropeptide AF.1 NPFF is expressed primarily in the posterior pituitary, hypothalamus, and medulla. It is an agonist at NPFF1 and NPFF2 receptors (Kis = 2.82 and 0.21 nM, respectively, for human recombinant receptors) that inhibits forskolin-induced cAMP production in CHO cells (EC50s = 236 and 2.3 nM, respectively).2 NPFF activates parvocellular neurons in the paraventricular nucleus (PVN) of the hypothalamus to stimulate oxytocin release from their projections in the brain stem, thereby regulating baroreflex control of heart rate.3 However, it inhibits magnocellular PVN neurons by enhancing GABAergic input. It is also found in plasma and exogenous administration briefly increases mean arterial pressure (MAP) by 40 mm Hg in rats, an effect that is only partially blocked by the norepinephrine competitor guanethidine (Item No. 16217) and the α1-adrenergic receptor antagonist prazosin (Item No. 15023).4 NPFF has anti-opioid effects in rodent models, inhibiting morphine-induced analgesia and inducing abstinence in morphine-tolerant rats.1,5 It also inhibits acquisition of conditioned place preference to cocaine in rats when administered at doses of 10 and 20 nmol.6

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Panula, P., Aarnisalo, A.A., and Wasowicz, K. Neuropeptide FF, a mammalian neuropeptide with multiple functions. Prog. Neurobiol. 48(4-5), 461-487 (1996).

    2. Mollereau, C., Mazarguil, H., Marcus, D., et al. Pharmacological characterization of human NPFF1 and NPFF2 receptors expressed in CHO cells by using NPY Y1 receptor antagonists. Eur. J. Pharmacol. 451(3), 245-256 (2002).

    3. Jhamandas, J.H., and Goncharuk, V. Role of neuropeptide FF in central cardiovascular and neuroendocrine regulation. Front. Endocrinol. (Lausanne) 4:8, (2013).

    4. Roth, B.L., Disimone, J., Majane, E.A., et al. Elevation of arterial pressure in rats by two new vertebrate peptides FLFQPQRF-NH2 and AGEGLSSPFWSLAAPQRF-NH2 which are immunoreactive to FMRF-NH2 antiserum. Neuropeptides 10(1), 37-42 (1987).

    5. Liu, Q., Guan, X.-M., Martin, W.J., et al. Identification and characterization of novel mammalian neuropeptide FF-like peptides that attenuate morphine-induced antinociception. The Journal of Biological Chemisty 276(40), 36961-36969 (2001).

    6. Kotlinska, J., Pachuta, A., and Silberring, J. Neuropeptide FF (NPFF) reduces the expression of cocaine-induced conditioned place preference and cocaine-induced sensitization in animals. Peptides 29(6), 933-939 (2008).