Pure recombinant enzyme
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ATG5 (human recombinant)

Item No. 25381

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • APG5-like
  • hAPG5
  • Apoptosis-specific Protein
  • Autophagy Protein 5
  • Autophagy-related 5
Purity
≥70% estimated by SDS-PAGE
Source
Recombinant N-terminal histidine-tagged ATG5 purified from E. coli
Amino Acids
2-275
MW
34.5 kDa
Formulation (Request formulation change)
50 mM HEPES, pH 8.0, 150 mM sodium chloride, and 10% glycerol
UniProt Accession №
Q9H1Y0
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    100 µg

    $528

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    Product Description

    Autophagy-related 5 (ATG5), formerly known as apoptosis specific protein (ASP), is a protein that is essential to autophagosome elongation.1,2,3 ATG5 is covalently conjugated to the C-terminal glycine residue of ATG12 (ATG12-ATG5) and forms a non-covalent complex with ATG16 (ATG12-ATG5-ATG16), which functions as an E3 ubiquitin ligase-like enzyme to facilitate LC3 transfer from ATG3 to phosphatidylethanolamine in canonical autophagy. ATG12-ATG5 also binds to the ATG12-ATG5-interaction region of the lysosomal localized protein TECPR1, freeing the TECPR1 pleckstrin homology domain to interact with phosphatidylinositol 3-phosphate components in the autophagosome membrane, promoting autophagosome-lysosome fusion.3 Polymorphisms in ATG5 have been associated with various autoimmune diseases, including lupus nephritis and Behcet's disease, gastrointestinal and colorectal cancers, as well as sporadic Parkinson's disease and childhood asthma.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Otomo, C., Metlagel, Z., Takaesu, G., et al. Structure of the human ATG12~ATG5 conjugate required for LC3 lipidation in autophagy. Nat. Struct. Mol. Biol. 20(1), 59-66 (2013).

    2. Kim, J.H., Hong, S.B., Lee, J.K., et al. Insights into autophagosome maturation revealed by the structures of ATG5 with its interacting partners. Autophagy 11(1), 75-87 (2015).

    3. Ye, X., Zhou, X.J., and Zhang, H. Exploring the role of autophagy-related gene 5 (ATG5) yields important insights into autophagy in autoimmune/autoinflammatory diseases. Front. Immunol. 9, 2334 (2018).