For immunochemical detection of ATG5
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25381ATG5 (human recombinant)
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ATG5 Monoclonal Antibody (Clone 4D5)

Item No. 26671

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • APG5-like
  • hAPG5
  • Apoptosis-specific Protein
  • Autophagy Protein 5
  • Autophagy-related 5
Immunogen
Full-length human recombinant ATG5 protein
Clone Designation
4D5
Formulation (Request formulation change)
100 µg of Protein G-purified monoclonal antibody
Storage Buffer
PBS, pH 7.2, with 50% glycerol and 0.02% sodium azide
Host
Mouse
Isotype
IgG1
Applications
ELISA, IHC, and WB
Cross Reactivity
(-) ATG8
Species Reactivity
(+) Human; other species not tested
UniProt Accession №
Q9H1Y0
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    100 µg

    $334

    Add
    Cayman Chemical
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    Product Description

    Autophagy-related 5 (ATG5), formerly known as apoptosis-specific protein (ASP), is a protein that is essential to autophagosome elongation.1,2,3 ATG5 is covalently conjugated to the C-terminal glycine residue of ATG12 (ATG12-ATG5) and forms a non-covalent complex with ATG16 (ATG12-ATG5-ATG16), which functions as an E3 ubiquitin ligase-like enzyme to facilitate LC3 transfer from ATG3 to phosphatidylethanolamine in canonical autophagy. ATG12-ATG5 also binds to the ATG12-ATG5-interaction region of the lysosomally localized protein TECPR1, freeing the TECPR1 pleckstrin homology domain to interact with phosphatidylinositol 3-phosphate components in the autophagosome membrane, promoting autophagosome-lysosome fusion.3 Polymorphisms in ATG5 have been associated with various autoimmune diseases, including lupus nephritis and Behçet's disease, gastrointestinal and colorectal cancers, as well as sporadic Parkinson's disease and childhood asthma. Cayman’s ATG5 Monoclonal Antibody (Clone 4D5) can be used for ELISA, immunohistochemistry, and Western blot applications. The antibody recognizes the ATG5-ATG12 complex at approximately 56 kDa from human samples.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Otomo, C., Metlagel, Z., Takaesu, G., et al. Structure of the human ATG12~ATG5 conjugate required for LC3 lipidation in autophagy. Nat. Struct. Mol. Biol. 20(1), 59-66 (2013).

    2. Kim, J.H., Hong, S.B., Lee, J.K., et al. Insights into autophagosome maturation revealed by the structures of ATG5 with its interacting partners. Autophagy 11(1), 75-87 (2015).

    3. Ye, X., Zhou, X.J., and Zhang, H. Exploring the role of autophagy-related gene 5 (ATG5) yields important insights into autophagy in autoimmune/autoinflammatory diseases. Front. Immunol. 9, 2334 (2018).