A bisdeoxy derivative of coelenterazine
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Alternative(s)
16157Coelenterazine 400a
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Product Categories

Product Type

Biochemicals
Labeling & Detection
Fluorescent Probes

Research Area

Application

Fluorescence

Coelenterazine 400a (hydrochloride)

Item No. 25738

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
6-phenyl-2,8-bis(phenylmethyl)-imidazo[1,2-a]pyrazin-3(7H)-one, monohydrochloride
CAS Number
2320429-05-6
Molecular Formula
C26H21N3O • HCl
Formula Weight
427.9
Purity
≥95%
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 0.5 mg/mlDMSO: 0.5 mg/mlEthanol: Slightly soluble
λmax
246, 434 nm
SMILES
O=C1C(CC2=CC=CC=C2)=NC3=C(CC4=CC=CC=C4)NC(C5=CC=CC=C5)=CN31.Cl
InChi Code
InChI=1S/C26H21N3O.ClH/c30-26-23(17-20-12-6-2-7-13-20)28-25-22(16-19-10-4-1-5-11-19)27-24(18-29(25)26)21-14-8-3-9-15-21;/h1-15,18,27H,16-17H2;1H
InChi Key
OCNXJLISLYDIAG-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

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    500 µg

    $90

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    1 mg

    $158

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    5 mg

    $696

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    Product Description

    Coelenterazine 400a is a bisdeoxy derivative of coelenterazine (Item No. 16123) that displays an emission maximum of 395 nm following conversion by Renilla luciferase (Rluc).1,2 It is used in bioluminescence resonance energy transfer 2 (BRET2) protocols, whereas coelenterazine h (Item No. 16894), which displays an emission maximum of 475 nm upon conversion by Rluc, is used in BRET1 protocols.3 Coelenterazine 400a is commonly paired with class 1 and 3 GFP acceptors, including GFP2 and GFP10.2,3 BRET2 assays are commonly used in evaluating protein-protein interactions, including those involved in G protein-coupled receptor signaling.1,2,4

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Bertrand, L., Parent, S., Caron, M., et al. The BRET2/arrestin assay in stable recombinant cells: A platform to screen for compounds that interact with G protein-couple receptors (GPCRS). J. Recept. Signal Transduct. Res. 22(1-4), 533-541 (2002).

    2. Galés, C., Rebois, R.V., Hogue, M., et al. Real-time monitoring of receptor and G-protein interactions in living cells. Nat. Methods 2(3), 177-184 (2005).

    3. Pfleger, K.D., and Eidne, K.A. Illuminating insights into protein-protein interactions using bioluminescence resonance energy transfer (BRET). Nat. Methods 3(3), 165-174 (2006).

    4. Huang, Q., Acha, V., Yow, R., et al. Bioluminescence measurements in mice using a skin window. J. Biomed. Opt. 12(5):054012, (2007).