Pure recombinant enzyme
Technical Support & Resources

Visit our FAQ

Contact Us

Toll Free Phone (USA and Canada Only): (888) 526-5351
Direct Phone: (734) 975-3888

Request Technical Support

Technical Support Request

To streamline the process attach the appropriate questionnaire to your inquiry.

Download IHC QuestionnaireDownload WB Questionnaire

View Our Privacy Statement for details on how we use and protect your data. In addition, this site is protected by hCaptcha and its Privacy Policy and Terms of Service apply.

MAP1LC3B/LC3B (human recombinant)

Item No. 25829

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Autophagy-related Protein LC3B
  • Autophagy-related Ubiquitin-like Modifier LC3B
  • MAP1 Light Chain 3-like Protein 2
  • Microtubule-associated Protein 1 Light Chain 3β
Purity
≥95% estimated by SDS-PAGE
Source
Recombinant N-terminal His-tagged MAP1LC3B/LC3B purified from E. coli
Amino Acids
2-120
MW
16.22 kDa
Formulation (Request formulation change)
50 mM HEPES, pH 8.0, with 150 mM sodium chloride, and 10% glycerol
UniProt Accession №
Q9GZQ8
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
Recommended Products

Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

Download most recent Certificate of Analysis for this product

    100 µg

    $321

    Add
    Cayman Chemical
    Visit Our Cancer Resource Center
    Find Tools & Resources to Study the Hallmarks of Cancer
    • Cancer cell signaling & regulation
    • Cancer metabolism
    • Tumor microenvironment
    EXPLORE NOW
    Product Description

    Microtubule-associated protein 1 light chain 3β (MAP1LC3B), also known as LC3B, is an ortholog of yeast Atg8 that is essential to autophagosome biogenesis.1 LC3B is irreversibly cleaved by the cysteine protease ATG4B (Item No. 25380) to its active form LC3B-I, which contains an exposed C-terminal glycine residue needed for autophagosome formation.2,3 LC3B-I is then conjugated to phosphatidylethanolamine in canonical autophagy by the ATG12-ATG5-ATG16 complex to form LC3B-II, which localizes to the autophagosomal membrane.1,4,5,6 Knockdown of LC3B increases the number of ATG5- and ATG16-positive puncta in HeLa cells, indicating a defect in the maturation of autophagosomes.1 LC3B expression is upregulated in various cancers and has disease-specific implications on progression and prognosis.7,8 In metastatic and castration-resistant human prostate cancer tumor samples, LC3B expression is upregulated and is positively correlated with reduced disease-specific mortality.7 Expression of LC3B is also increased in oral squamous cell carcinomas and is associated with aggressive pathological features and poor prognosis.8

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Weidberg, H., Shvets, E., Shpilka, T., et al. LC3 and GATE-16/GABARAP subfamilies are both essential yet act differently in autophagosome biogenesis. EMBO J. 29(11), 1792-1802 (2010).

    2. Li, M., Hou, Y., Wang, J., et al. Kinetics comparisons of mammalian Atg4 homologues indicate selective preferences toward diverse Atg8 substrates. The Journal of Biological Chemisty 286(9), 7327-7338 (2011).

    3. Yang, Z., Wilkie-Grantham, R.P., Yanagi, T., et al. ATG4B (Autophagin-1) phosphorylation modulates autophagy. The Journal of Biological Chemisty 290(44), 26549-26561 (2015).

    4. Kim, J.H., Hong, S.B., Lee, J.K., et al. Insights into autophagosome maturation revealed by the structures of ATG5 with its interacting partners. Autophagy 11(1), 75-87 (2015).

    5. Ye, X., Zhou, X.J., and Zhang, H. Exploring the role of autophagy-related gene 5 (ATG5) yields important insights into autophagy in autoimmune/autoinflammatory diseases. Front. Immunol. 9, 2334 (2018).

    6. Mortezavi, A., Salemi, S., Rupp, N.J., et al. Negative LC3b immunoreactivity in cancer cells is an independent prognostic predictor of prostate cancer specific death. Oncotarget 8(19), 31765-31774 (2017).

    7. Liu, J.-L., Chen, F.-F., Lung, J., et al. Prognostic significance of p62/SQSTM1 subcellular localization and LC3B in oral squamous cell carcinoma. Br. J. Cancer 111(5), 944-954 (2014).