Pure human recombinant enzyme
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Measurement and Detection Target(s)
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METTL16 (human, recombinant)

Item No. 26343

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • EC 2.1.1.346
  • EC 2.1.1.62
  • Methyltransferase-Like Protein 16
  • Methyltransferase 10 Domain-Containing Protein
  • N6-Adenosine-Methyltransferase METTL16
  • Putative Methyltransferase METT10D
  • U6 Small Nuclear RNA (adenine-(43)-N(6))-Methyltransferase
  • U6 snRNA Methyltransferase METT10D
Purity
≥90% estimated by SDS-PAGE
Source
Human recombinant C-terminal histidine-tagged METTL16 purified from E. coli
Amino Acids
2-562 (full length)
MW
64.5 kDa
Formulation (Request formulation change)
50 mM HEPES, pH 8.0, with 150 mM sodium chloride and 10% glycerol
UniProt Accession №
Q86W50
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    100 µg

    $528

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    Product Description

    Methyltransferase-like protein 16 (METTL16) is an m6A RNA methyltransferase encoded by the METTL16 gene in humans.1 It is composed of a methyltransferase domain that associates with RNA and two vertebrate conserved regions (VCRs) on the C-terminal end that promote splicing.2 METTL16 acts as an epigenetic writer by methylating the pre-mRNA of the S-adenosylmethionine (SAM) synthetase MAT2A in the presence of SAM. It also methylates the adenine at position 43 of spliceosome component U6 snRNA and interacts with cancer-associated MALAT1 long non-coding RNA (lncRNA).3,1 Knockout of Mettl16 in mouse embryos leads to a reduction in Mat2a mRNA expression, transcriptome dysregulation, and lethality at approximately the implantation stage.4 The expression of METTL16 is increased in isolated human colon, but not rectal, adenocarcinoma tumor tissue, and decreased METTL16 expression in rectal adenocarcinoma tumor tissue is associated with lower overall survival.5

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Ruszkowska, A., Ruszkowski, A., Dauter, Z., et al. Structural insights into the RNA methyltransferase domain of METTL16. Sci. Rep. 8(1), 5311 (2018).

    2. Pendleton, K.E., Chen, B., Liu, K., et al. The U6 snRNA m6A methyltransferase METTL16 regulates SAM synthetase intron retention. Cell 169(5), 824-835 (2017).

    3. Warda, A.S., Kretschmer, J., Hackert, P., et al. Human METTL16 is a N6-methyladenosine (m6A) methyltransferase that targets pre-mRNAs and various non-coding RNAs. EMBO Rep. 18(11), 2004-2014 (2017).

    4. Mendel, M., Chen, K.M., Homolka, D., et al. Methylation of structured RNA by the m6A writer METTL16 is essential for mouse embryonic development. Mol. Cell. 71(5), 986-1000 (2018).

    5. Liu, X., Liu, L., Dong, Z., et al. Expression patterns and prognostic value of m6A-related genes in colorectal cancer. Am. J. Transl. Res. 11(7), 3972-3991 (2019).