For immunochemical detection of FADS1
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Fatty Acid Desaturase 1 Monoclonal Antibody (Clone 3A10.1)

Item No. 27533

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Acyl-CoA (8-3)-Desaturase
  • FADS1
  • Δ5 Fatty Acid Desaturase
Immunogen
Recombinant human FADS1 protein
Clone Designation
3A10.1
Formulation (Request formulation change)
300 µg of protein G-purified monoclonal antibody
Storage Buffer
PBS, pH 7.2, with 50% glycerol and 0.02% sodium azide
Host
Mouse
Isotype
IgG1
Applications
ELISA and WB
Cross Reactivity
(+) FADS1(-) FADS2
Species Reactivity
(+) Human; other species not tested
UniProt Accession №
O60427
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    300 µg

    $440

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    Product Description

    Fatty acid desaturase 1 (FADS1), also known as Δ5 desaturase, is a 444-amino acid transmembrane protein encoded by FADS1.1 It catalyzes conversion of dihomo-γ-linolenic acid (Item No. 90230) to arachidonic acid (Item Nos. 90010 | 90010.1 | 10006607) and ω-3 arachidonic acid (Item No. 90011) to eicosapentaenoic acid (Item Nos. 90110 | 90110.1 | 21908) during the production of ω-6 and ω-3 long-chain polyunsaturated fatty acids (LC-PUFAs), respectively. Hepatic FADS1 protein levels and mRNA expression are increased in mice with high-fat diet-induced obesity and non-alcoholic steatohepatitis (NASH).2 Tissue-selective knockdown of Fads1 in the liver, adipose tissue, and reticuloendothelial system of adult hyperlipidemic LDL receptor-null mice promotes hepatic inflammation and formation of atherosclerotic plaques and suppresses hepatic lipogenesis.3 Increased expression of FADS1 positively correlates with disease-free survival and overall survival times in patients with esophageal squamous cell carcinoma (ESCC).4 Cayman's Fatty Acid Desaturase 1 Monoclonal Antibody (Clone 3A10.1) can be used for immunoprecipitation (IP), ELISA, and Western blot (WB) applications. The antibody recognizes FADS1 at approximately 45 kDa from human samples.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Lattka, E., Illig, T., Heinrich, J., et al. FADS gene cluster polymorphisms: Important modulators of fatty acid levels and their impact on atopic diseases. J. Nutrigenet. Nutrigenomics 2(3), 119-128 (2009).

    2. López-Vicario, C., González-Périz, A., Rius, B., et al. Molecular interplay between Δ5/Δ6 desaturases and long-chain fatty acids in the pathogenesis of non-alcoholic steatohepatitis. Gut 63(2), 344-355 (2014).

    3. Gromovsky, A.D., Schugar, R.C., Brown, A.L., et al. The Δ-5 fatty acid desaturase FADS1 impacts metabolic disease by balancing pro-inflammatory and pro-resolving lipid mediators. Arterisoscler. Thromb. Vasc. Biol. 38(1), 218-231 (2018).

    4. Du, Y., Yan, S.M., Gu, W.Y., et al. Decreased expression of FADS1 predicts a poor prognosis in patients with esophageal squamous cell carcinoma. Asian Pac. J. Cancer Prev. 16(12), 5089-5094 (2015).