An amine- and thiol-reactive fluorescent probe
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Isomer(s)
33264Fluorescein 5(6)-isothiocyanate
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Fluorescein 5-isothiocyanate

Item No. 27814

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
3',6'-dihydroxy-5-isothiocyanato-spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one
CAS Number
3326-32-7
Synonyms
  • 5-FITC
  • FITC isomer I
  • 5-Isothiocyanatofluorescein
Molecular Formula
C21H11NO5S
Formula Weight
389.4
Purity
≥90%
Emission
519 nm
Excitation
494 nm
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMF: 14 mg/mlDMSO: 14 mg/mlEthanol: 10 mg/mlPBS (pH 7.2): 0.2 mg/ml
λmax
226, 278 nm
SMILES
OC1=CC=C(C2(C(C=CC(N=C=S)=C3)=C3C(O2)=O)C(C=CC(O)=C4)=C4O5)C5=C1
InChi Code
InChI=1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H
InChi Key
MHMNJMPURVTYEJ-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    250 mg

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    Product Description

    Fluorescein 5-isothiocyanate (5-FITC) is an isomer of fluorescein isothiocyanate (FITC), which is commonly used as a mixture of the 5- and 6-isothiocyanate isomers.1,2 It reacts with amine and thiol groups to form conjugates with proteins, lipids, and other molecules for detection by a variety of fluorescent-based applications.1,3 5-FITC displays excitation/emission maxima of 494/519 nm, respectively.3

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Schnaible, V., and Przybylski, M. Identification of fluorescein-5'-isothiocyanate-modification sites in proteins by electrospray-ionization mass spectrometry. Bioconjug. Chem. 10(5), 861-866 (1999).

    2. Hunkapiller, M.W., Connell, C.R., Mordan, W.J., et al. Real time scanning electrophoresis apparatus for DNA sequencing. (1986).

    3. Sabnis, R.W. Fluoroscein-5-isothiocyanate (FITC). Handbook of Fluorescent Dyes and Probes 219 (2015).