An amine-reactive fluorescent probe
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Isomer(s)
27814Fluorescein 5-isothiocyanate
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Fluorescein 5(6)-isothiocyanate

Item No. 33264

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
3′,6′-dihydroxy-5(or 6)-isothiocyanato-spiro[isobenzofuran-1(3H),9′-[9H]xanthen]-3-one
CAS Number
27072-45-3
Synonyms
  • FITC
  • Fluorescein isothiocyanate
Molecular Formula
C21H11NO5S
Formula Weight
389.4
Purity
≥90% (mixture of isomers)
Emission
535 nm
Excitation
485 nm
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 10 mg/mlDMF:PBS (pH 7.2) (1:2): 0.33 mg/mlDMSO: 5 mg/mlEthanol: Slightly solublePBS (pH 7.2): Slightly soluble
λmax
224 nm
SMILES
OC1=CC=C2C(OC3=CC(O)=CC=C3C24C(C=CC(N=C=S)=C5)=C5C(O4)=O)=C1.OC6=CC=C7C(OC8=CC(O)=CC=C8C79C(C=C(N=C=S)C=C%10)=C%10C(O9)=O)=C6
InChi Code
InChI=1S/2C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21;23-12-2-5-15-18(8-12)26-19-9-13(24)3-6-16(19)21(15)17-7-11(22-10-28)1-4-14(17)20(25)27-21/h2*1-9,23-24H
InChi Key
AEWCEHLYVLRLBF-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

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    Product Description

    Fluorescein 5(6)-isothiocyanate (FITC) is an amine-reactive fluorescent probe.1,2 It reacts with amine groups to form conjugates with proteins for detection by various fluorescent-based applications. FITC displays excitation/emission maxima of 485/535 nm, respectively.2

    WARNING This product is not for human or veterinary use.