For immunochemical detection of PINK1
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Alternative(s)
10006283PINK1 Polyclonal Antibody
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PINK1 Monoclonal Antibody (Clone 3E8)

Item No. 29129

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • BRPK
  • PARK6
  • PTEN Induced Putative Kinase 1
  • PTEN Inducible Kinase 1
  • Serine/Threonine-Protein Kinase PINK1
Immunogen
Recombinant human PINK1 amino acids 156-511
Clone Designation
3E8
Formulation (Request formulation change)
200 µg protein A-purified monoclonal antibody
Storage Buffer
PBS, pH 7.2, with 50% glycerol and 0.02% sodium azide
Host
Mouse
Isotype
IgG2a
Applications
WB and ELISA
Cross Reactivity
(+) PINK1
Species Reactivity
(+) Human
UniProt Accession №
Q9BXM7
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    200 µg

    $440

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    Product Description

    PTEN-induced putative kinase 1 (PINK1) is a serine/threonine protein kinase that has a role in mitochondrial function.1,2 It is comprised of an N-terminal mitochondrial targeting sequence, a transmembrane domain, a serine/threonine kinase domain, and a C-terminal region.2 PINK1 is ubiquitously expressed primarily in the brain, skeletal muscle, and heart.3 It localizes to the mitochondria where it is either rapidly degraded or, under conditions of low mitochondrial membrane potential, accumulates on the outer mitochondrial membrane, where it recruits and activates the cytosolic E3 ubiquitin ligase Parkin, which targets the mitochondria for mitophagy.1,3 Pink1 knockout in rats leads to an age-dependent loss of dopaminergic neurons in the substantia nigra, as well as deficits in motor function and mitochondrial respiration.4 In mice, Pink1 knockout does not induce a loss of dopaminergic neurons without concomitant overexpression of α-synuclein in the substantia nigra.5 Loss-of-function mutations in PINK1 are causally associated with autosomal recessive early-onset Parkinson’s disease.3,6 Cayman’s PINK1 Monoclonal Antibody (Clone 3E8) recognizes primarily the full length protein at about 66 kDa in human tissues. This antibody can be used for ELISA and Western blot applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Trempe, J.-F., and Fon, E.A. Structure and function of parkin, PINK1, and DJ-1, the three musketeers of neuroprotection. Front. Neurol. 4, 38 (2013).

    2. Sim, C.H., Gabriel, K., Mills, R.D., et al. Analysis of the regulatory and catalytic domains of PTEN-induced kinase-1 (PINK1). Hum. Mutat. 33(10), 1408-1422 (2012).

    3. Barodia, S.K., Creed, R.B., and Goldberg, M.S. Parkin and PINK1 functions in oxidative stress and neurodegeneration. Brain Res. Bull. 133, 51-59 (2017).

    4. Creed, R.B., and Goldberg, M.S. New developments in genetic rat models of Parkinson’s disease. Mov. Disord. 33(5), 717-729 (2018).

    5. Oliveras-Salvá, M., Macchi, F., Coessens, V., et al. Alpha-synuclein-induced neurodegeneration is exacerbated in PINK1 knockout mice. Neurobiol. Aging 35(11), 2625-2636 (2014).

    6. Valente, E.M., Abou-Sleiman, P.M., Caputo, V., et al. Hereditary early-onset Parkinson’s disease caused by mutations in PINK1. Science 304(5674), 1158-1160 (2004).