For immunochemical detection of AC3
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Adenylate Cyclase Type 3 Polyclonal Antibody

Item No. 29250

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • ACIII
  • Adenylyl Cyclase 3
  • Adenylate Cyclase III
  • Adenylyl Cyclase Type III
Immunogen
Synthetic peptide corresponding to the C-terminal region of rat adenylate cyclase type 3 conjugated to KLH
MW
~160 kDa
Formulation (Request formulation change)
100 µl of affinity-purified polyclonal antibody
Storage Buffer
PBS with 5 mM sodium azide and 50% glycerol
Host
Rabbit
Applications
WB, IHC, ICC
Species Reactivity
(+) Human(+) Mouse(+) Rat
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

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    100 µl

    $534

    Add
    Cayman Chemical
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    Product Description

    Adenylate cyclase type 3 (AC3) is a membrane-bound protein that catalyzes the formation of the second messenger cAMP from ATP (Item No. 14498) in response to G protein-coupled receptor (GPCR) signaling.1,2 It consists of 1,144 amino acids and is comprised of a cytoplasmic N-terminus followed by two repeating modules, each consisting of a transmembrane domain and a cytoplasmic domain, with the transmembrane domains composed of six membrane-spanning helices apiece.3,1 ADCY3, the gene encoding AC3, is expressed in a variety of tissues, with high levels in the placenta, testes, ovaries, and colon in humans.1,3 AC3 also localizes to primary cilia in the brain and has been used as a marker of primary cilia in mouse brain.4,5 Mutations in ADCY3 are associated with an increased risk of obesity and type 2 diabetes.2,3,4 Cayman's Adenylate Cyclase Type 3 Polyclonal Antibody can be used for immunocytochemistry (ICC), immunohistochemistry (IHC), and Western blot (WB) applications. The antibody recognizes AC3 at approximately 160 kDa from human, mouse, and rat samples.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Ludwig, M.-G., and Seuwen, K. Characterization of the human adenylyl cyclase gene family: cDNA, gene structure, and tissue distribution of the nine isoforms. J. Recept. Signal Transduct. Res. 22(1-4), 79-110 (2002).

    2. Grarup, N., Moltke, I., Andersen, M.K., et al. Loss-of-function variants in ADCY3 increase risk of obesity and type 2 diabetes. Nat. Genet. 50(2), 172-174 (2018).

    3. Saeed, S., Bonnefond, A., Tamanini, F., et al. Loss-of-function mutations in ADCY3 cause monogenic severe obesity. Nat. Genet. 50(2), 175-179 (2018).

    4. Barroso, I. ADCY3, neuronal primary cilia and obesity. Nat. Genet. 50(2), 166-167 (2018).

    5. Bishop, G.A., Berbari, N.F., Lewis, J., et al. Type III adenylyl cyclase localizes to primary cilia throughout the adult mouse brain. J. Comp. Neurol. 505(25), 562-571 (2007).