Active • Host: E. coli • AA: 2-373 • Tag: N-terminal His • MW: 42.5 kDa
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Measurement and Detection Target(s)
31406FSP1 Polyclonal Antibody
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FSP1 (human, recombinant)

Item No. 29611

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • AIFM2
  • Apoptosis-inducing Factor Homologous Mitochondrion-associated Inducer of Death
  • Apoptosis-inducing Factor Mitochondria-associated 2
  • Ferroptosis Suppressor Protein 1
  • p53-Responsive Gene 3 Protein
  • PRG3
Purity
≥70% estimated by SDS-PAGE
Source
Active recombinant N-terminal His-tagged human FSP1 expressed in E. coli
Amino Acids
2-373
MW
42.5 kDa
Formulation (Request formulation change)
50 mM sodium phosphate, pH 7.6, with 200 mM sodium chloride, 0.1 mM TECP, and 10% glycerol
UniProt Accession №
Q9BRQ8
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    50 µg

    $569

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    Cayman Chemical
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    Product Description

    Ferroptosis suppressor protein 1 (FSP1), also known as apoptosis-inducing factor mitochondria-associated 2 (AIFM2), is a flavoprotein and NAD(P)H-dependent oxidoreductase that inhibits ferroptosis in a glutathione peroxidase 4- and glutathione-independent manner.1,2,3 It is encoded by AIFM2 in humans and is composed of a short N-terminal hydrophobic region followed by a flavin adenine dinucleotide-dependent oxidoreductase domain.2 FSP1 localizes to the plasma membrane in a myristoylation-dependent manner, where it reduces coenzyme Q10 (CoQ10; Item No. 11506) to CoQ10H2. Reduced CoQ10 functions as a radical-trapping antioxidant and inhibits lipid peroxidation. AIFM2 expression positively correlates with resistance to glutathione peroxidase 4 (GPX4) inhibitors, including (1S,3R)-RSL3 (Item No. 19288), ML-210 (Item No. 23282), and ML-162 (Item No. 20455) in cancer cell lines. Withdrawal of the ferroptosis inhibitor ferrostatin-1 (Item No. 17729) reduces tumor growth in an Fsp1/Gpx4 double knockout, but not a Gpx4 single knockout, H460 lung cancer mouse xenograft model, indicating that FSP1 confers resistance to ferroptotic cell death. Cayman's FSP1 (human, recombinant) protein can be used for enzyme assay applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Doll, S., Freitas, F.P., Shah, R., et al. FSP1 is a glutathione-independent ferroptosis suppressor. Nature 575(7784), 693-698 (2019).

    2. Bersuker, K., Hendricks, J., Li, Z., et al. The CoQ oxidoreductase FSP1 acts parallel to GPX4 to inhibit ferroptosis. Nature 575(7784), 688-692 (2019).

    3. Marshall, K.R., Gong, M., Wodke, L., et al. The human apoptosis-inducing protein AMID is an oxidoreductase with a modified flavin cofactor and DNA binding activity. The Journal of Biological Chemisty 280(35), 30735-30740 (2005).