A cell-impermeable fluorescent pH probe
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8-Hydroxypyrene-1,3,6-trisulfonic Acid (sodium salt)

Item No. 30080

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
8-hydroxy-1,3,6-pyrenetrisulfonic acid, trisodium salt
CAS Number
6358-69-6
Synonyms
  • HPTS
  • Pyranine
Molecular Formula
C16H7O10S3 • 3Na
Formula Weight
524.4
Purity
≥95%
Emission
510 nm
Excitation
403 and 450 nm, the intensities of which decrease and increase, respectively, as pH increases from 5 to 9
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 1 mg/mlDMSO: 15 mg/mlPBS (pH 7.2): 10 mg/ml
λmax
247, 290, 370, 404 nm
SMILES
[O-]S(C1=CC(S([O-])(=O)=O)=C2C3=C4C(C=C2)=C(S([O-])(=O)=O)C=C(O)C4=CC=C31)(=O)=O.[Na+].[Na+].[Na+]
InChi Code
InChI=1S/C16H10O10S3.3Na/c17-11-5-12(27(18,19)20)8-3-4-10-14(29(24,25)26)6-13(28(21,22)23)9-2-1-7(11)15(8)16(9)10;;;/h1-6,17H,(H,18,19,20)(H,21,22,23)(H,24,25,26);;;/q;3*+1/p-3
InChi Key
KXXXUIKPSVVSAW-UHFFFAOYSA-K
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
Certificates of Analysis & Batch Specific Data

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    500 mg

    $65

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    1 g

    $106

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    5 g

    $493

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    Cayman Chemical
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    Product Description

    8-Hydroxypyrene-1,3,6-trisulfonic acid (HPTS) is a cell-impermeable fluorescent pH probe.1,2 It displays excitation maxima of 403 and 450 nm, the intensities of which decrease and increase, respectively, as pH increases from 5 to 9 and an emission maximum of 510 nm.1 HPTS has been used in excitation ratio imaging to monitor changes in and measure the pH of endocytosed liposomes, acidic organelles, or the cytoplasm in live cell applications.1,2 It is sensitive to fluorescence quenching by viologens, which can be reversed in the presence of glucose or other monosaccharides, and has been used as a glucose and carbohydrate sensor.3

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Daleke, D.L., Hong, K., and Papahadjopoulos, D. Endocytosis of liposomes by macrophages: binding, acidification and leakage of liposomes monitored by a new fluorescence assay. Biochim. Biophys. Acta 1024(2), 352-366 (1990).

    2. Han, J., and Burgess, K. Fluorescent indicators for intracellular pH. Chem. Revs. 110(5), 2709-2728 (2010).

    3. Cordes, D.B., and Singaram, B. A unique, two-component sensing system for fluorescence detection of glucose and other carbohydrates. Pure Appl. Chem. 84(11), 2183-2202 (2012).