Host: HEK293 cells • AA: 30-212 • Tag: N-terminal His • MW: 20.9 kDa
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Measurement and Detection Target(s)
36178Citrullinated Interleukin-6 Polyclonal Antibody
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Citrullinated Interleukin-6 (human, recombinant)

Item No. 30173

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • B Cell Stimulatory Factor 2
  • BSF-2
  • CDF
  • CTL Differentiation Factor
  • HGF
  • Hybridoma Growth Factor
  • IFN-β2
  • IL-6
  • Interferon-β2
Purity
≥85% estimated by SDS-PAGE
Endotoxin Testing
<1 EU/µg determined by the LAL assay
Source
Recombinant human N-terminal His-tagged IL-6 expressed in HEK293 cells, citrullinated by PAD4
Amino Acids
30-212
MW
20.9 kDa
Formulation (Request formulation change)
50 mM HEPES, pH 8.0, with 150 mM sodium chloride, 10% glycerol, 2 mM β-mercaptoethanol
UniProt Accession №
P05231
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

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    100 µg

    $600

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    Product Description

    Interleukin-6 (IL-6) is a cytokine encoded by the IL6 gene in humans with roles in both the initiation and resolution of inflammatory responses.1,2 IL-6 is produced in response to an inflammatory event, induces recruitment of neutrophils to the site of inflammation, and signals through the membrane-bound IL-6 receptor (IL-6R) to upregulate the synthesis of acute phase proteins, lipolysis in the liver, and induced differentiation of T and B cells. Following initiation of this classical signaling pathway, a soluble form of IL-6R (sIL-6R) is released from neutrophils to promote recruitment of monocytes and macrophages, induction of fever via IL-6 action in the hypothalamus, and production of anti-inflammatory M2 macrophages to initiate tissue repair. Production of IL-6 is dysregulated in various chronic inflammatory diseases, including rheumatoid arthritis, Castleman disease, polymyalgia rheumatica, and giant cell arteritis.3 It also induces tumor growth and metastasis via activation of JAK/STAT signaling.4 Recombinant human IL-6 can be citrullinated at arginine residues by protein arginine deiminase 4 (PAD4) in vitro.5 Citrullinated IL-6 and anti-citrullinated peptide antibodies (ACPAs) reactive with citrullinated IL-6 have been found in synovial fluid and sera, respectively, from patients with rheumatoid arthritis. This product contains purified IL-6 (human, recombinant) (Item No. 30174) that has been modified with PAD4 enzyme, which is subsequently depleted by affinity chromatography.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Del Giudice, M., and Gangestad, S.W. Rethinking IL-6 and CRP: Why they are more than inflammatory biomarkers, and why it matters. Brain Behav. Immun. 70, 61-75 (2018).

    2. Rose-John, S. Interleukin-6 family cytokines. Cold Spring Harb. Perspect. Biol. 10(2), a028415 (2018).

    3. Schett, G. Physiological effects of modulating the interleukin-6 axis. Rheumatology (Oxford) 57(suppl_2), ii43-ii50 (2018).

    4. Lacina, L., Brábek, J., Král, V., et al. Interleukin-6: A molecule with complex biological impact in cancer. Histol. Histopathol. 34(2), 125-136 (2019).

    5. Lu, C., Ohara, R., Campbell, P., et al. Citrullination of interleukin 6 augments its pro-inflammatory capacity and signaling potency through interleukin-6 receptor in rheumatoid arthritis [abstract]. Arthritis Rheumatol. 71 (suppl 10), (2019).