EP₂ Receptor Blocking Peptide

Each vial contains 200 µg of lyophilized peptide derived from the human EP₂ receptor sequence (C-terminal amino acids 335-358; SLRTQDATQTSCSTQSDASKQADL).¹ This peptide was used as an antigen for production of the EP₂ receptor polyclonal antibody (Catalog No. 101750) and can be used in conjunction with this antibody to block protein-antibody complex formation during immunochemical analysis for the EP₂ receptor. The biological effects of prostaglandin E₂ (PGE₂) are mediated through interaction with four distinct membrane-bound G-protein coupled EP receptors: EP₁, EP₂, EP₃, and EP₄.^2,3 Binding of PGE₂ to the EP₂ receptor results in an increase in adenylate cyclase activity with a subsequent increase in cAMP.^1,4 Pharmacologically, EP₂ receptors mediate relaxation of smooth muscle and are distinguished from EP₄ receptors by their sensitivity to the EP₂ receptor selective agonist butaprost.^2,3,1 The human EP₂ receptor is comprised of 358 amino acids with a molecular mass of approximately 40,000.¹ A band at 53 kDa is detected in most samples by western blotting. mRNA for the EP₂ receptor is expressed in a variety of tissues including lung, placenta, spleen, intestine, kidney, and sensory neuron.^1,4,5

WARNING This product is not for human or veterinary use.

1. Regan, J.W., Bailey, T.J., Pepperl, D.J., et al. Cloning of a novel human prostaglandin receptor with characteristics of the pharmacologically defined EP₂ subtype. Mol. Pharmacol. 46(2), 213-220 (1994).

2. Coleman, R.A., Smith, W.L., and Narumiya, S. International Union of Pharmacology classification of prostanoid receptors: Properties, distribution, and structure of the receptors and their subtypes. Pharmacol. Rev. 46(2), 205-229 (1994).

3. Coleman, R.A., Dahlén, S.E., Drazen, J.M., et al. Classification of leukotriene receptors IUPHAR receptor compendium. IUPHAR Compendium 1-7 (1997).

4. Nemoto, K., Pilbeam, C.C., Bilak, S.R., et al. Molecular cloning and expression of a rat prostaglandin E₂ receptor of the EP₂ subtype. Prostaglandins 54(4), 713-725 (1997).

5. Southall, M.D., and Vasko, M.R. Prostaglandin receptor subtypes, EP3C and EP₄, mediate the prostaglandin E₂-induced cAMP production and sensitization of sensory neurons. The Journal of Biological Chemisty 276(19), 16083-16091 (2001).