Active • Host: Insect cells • AA: 2-654 (full length) • Tag: N-terminal His • MW: 72.9 kDa
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Measurement and Detection Target(s)
31096Sphingosine Kinase 2 Monoclonal Antibody (Clone 2C6)
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Sphingosine Kinase 2 (human, recombinant)

Item No. 30191

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • SK2
  • SPHK2
  • SPK2
Purity
≥75% estimated by SDS-PAGE
Source
Active recombinant N-terminal His-tagged protein expressed in insect cells
Amino Acids
2-654 (full length)
MW
72.9 kDa
Formulation (Request formulation change)
25 mM HEPES, pH 8.0, with 150 mM sodium chloride, 3 mM DTT, 0.05% Triton X-100, and 5% glycerol
UniProt Accession №
Q9NRA0
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    25 µg

    $469

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    Product Description

    Sphingosine kinase 2 (SPHK2) is an ATP-dependent lipid kinase that is encoded by the SPHK2 gene in humans.1 It is composed of an N-terminal domain that contains an ATP binding site and a C-terminal domain that mediates substrate binding and specificity. SPHK2 is localized to the mitochondria, nucleus, and endoplasmic reticulum and is expressed in a wide variety of tissues, including the liver and kidney.2 Upon cellular stimulation with EGF, phorbol 12-myristate 13-acetate (PMA; Item No. 10008014), or FcεRI, SPHK2 is activated and catalyzes phosphorylation of sphingosine to sphingosine-1-phosphate (S1P).1,3 SPHK2 has roles in many physiological and pathological processes, including cancer, inflammation, and neurodegenerative diseases.1,4,5,6,7 Pharmacological inhibition of SPHK2 decreases intracellular S1P levels in U937 cells, but genetic deletion or pharmacological inhibition of SPHK2 in mice increases blood S1P levels, an effect that has not been fully characterized but may be related to reduced S1P clearance in the blood in the absence of SPHK2 activity.8 Overexpression of SPHK2 increases intracellular calcium levels and induces apoptosis in NIH3T3 cancer cells and reduces LPS-induced increases in TNF-α and IL-6 levels in isolated human peripheral blood mononuclear cell-derived macrophages in vitro.4,5 SPHK2 activity is increased in postmortem frontal cortex from patients with Alzheimer's disease.7 Cayman's Sphingosine Kinase 2 (human, recombinant) protein can be used for Western blot, ELISA, and enzymatic assays.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Hatoum, D., Haddadi, N., Lin, Y., et al. Mammalian sphingosine kinase (SphK) isoenzymes and isoform expression: Challenges for SphK as an oncotarget. Oncotarget 8(22), 36898-36929 (2017).

    2. Liu, H., Sugiura, M., Nava, V.E., et al. Molecular cloning and functional characterization of a novel mammalian sphingosine kinase type 2 isoform. The Journal of Biological Chemisty 275(26), 19513-19520 (2000).

    3. Hait, N.C., Bellamy, A., Milstien, S., et al. Sphingosine kinase type 2 activation by ERK-mediated phosphorylation. The Journal of Biological Chemisty 282(16), 12058-12065 (2007).

    4. Maceyka, M., Sankala, H., Hait, N.C., et al. SphK1 and SphK2, sphingosine kinase isoenzymes with opposing functions in sphingolipid metabolism. The Journal of Biological Chemisty 280(44), 37118-37129 (2005).

    5. Weigert, A., von Knethen, A., Thomas, D., et al. Sphingosine kinase 2 is a negative regulator of inflammatory macrophage activation. Biochim. Biophys. Acta Mol. Cell Biol. Lipids 1864(9), 1235-1246 (2019).

    6. Samy, E.T., Meyer, C.A., Caplazi, P., et al. Cutting edge: Modulation of intestinal autoimmunity and IL-2 signaling by sphingosine kinase 2 independent of sphingosine 1-phosphate. J. Immunol. 179(9), 5644-5648 (2007).

    7. Takasugi, N., Sasaki, T., Suzuki, K., et al. BACE1 activity is modulated by cell-associated sphingosine-1-phosphate. J. Neurosci. 31(18), 6850-6857 (2011).

    8. Kharel, Y., Morris, E.A., Congdon, M.D., et al. Sphingosine kinase 2 inhibition and blood sphingosine 1-phosphate levels. J. Pharmacol. Exp. Ther. 355(1), 23-31 (2015).