Expression Profiling of Clinically Relevant CYPs Utilizing upcyte^® Hepatocytes Assay Kit

The reagents and materials provided in this assay kit for the expression profiling of clinically relevant CYPs are formatted to allow for two alternative cell culture setups. In one scenario 48 culture wells may be setup at two different times. In the other assay scenario 96 culture wells may be setup at one time. The kit includes two aliquots of upcyte® hepatocytes, donor 10-03, isolated from an adult Caucasian female. These hepatocytes are cryopreserved using INDIGO’s proprietary CryoMite™ process, which yields high cell viability post-thaw, and provides the convenience of immediately dispensing cells into assay plates. There is no need for intermediate treatment steps such as spin-and-rinse of cells, viability determinations, or cell titer adjustments prior to assay setup. During an overnight culture period for cell recovery the hepatocytes will form a confluent monolayer that is ready to receive treatment media containing the user’s test compounds.

In addition to two aliquots of upcyte® hepatocytes, the kit provides two cell culture-ready assay plates, optimized Cell Culture Medium (CCM) for use in all steps of the assay procedure (cell thawing, seeding, and preparation of treatment media), three reference compounds (rifampicin, β-naphthoflavone, and chenodeoxycholic acid (CDCA)) that activate one or more of the primary xenobiotic-sensing receptors PXR, CAR, AhR and FXR. Upon activation, these nuclear receptors modulate the expression of the clinically relevant CYP genes. Finally, included are seven sets of validated qPCR primers for quantifying drug-induced changes in the expression of CYP3A4, CYP1A1, CYP2B6, CYP2C8, CYP2C9, CYP2C19, and CYP2E1, as well as primers for ACTB (β-actin; the internal control used to normalize all CYP gene expression data).

[INDIGO Catalog No. UGE1003-48]

WARNING This product is not for human or veterinary use.